DNA assays for genetic discrimination of three Phragmites australis subspecies in the United States

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2023-03-08 DOI:10.1002/aps3.11512

Denise L. Lindsay, Xin Guan, Nathan E. Harms, James T. Cronin, Laura A. Meyerson, Richard F. Lance

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Abstract

Premise

To genetically discriminate subspecies of the common reed (Phragmites australis), we developed real-time quantitative (qPCR) assays for identifying P. australis subsp. americanus, P. australis subsp. australis, and P. australis subsp. berlandieri.

Methods and Results

Utilizing study-generated chloroplast DNA sequences, we developed three novel qPCR assays. Assays were verified on individuals of each subspecies and against two non-target species, Arundo donax and Phalaris arundinacea. One assay amplifies only P. australis subsp. americanus, one amplifies P. australis subsp. australis and/or P. australis subsp. berlandieri, and one amplifies P. australis subsp. americanus and/or P. australis subsp. australis. This protocol enhances currently available rapid identification methods by providing genetic discrimination of all three subspecies.

Conclusions

The newly developed assays were validated using P. australis samples from across the United States. Application of these assays outside of this geographic range should be preceded by additional testing.

Abstract Image

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美国三个芦苇亚种遗传鉴别的DNA分析

前提为了对普通芦苇（Phragmites australis）的亚种进行遗传鉴别，我们开发了实时定量（qPCR）方法来鉴定芦苇亚种。americanus，P.australis亚种。australis和P.australis亚种。berlandieri。方法和结果利用研究产生的叶绿体DNA序列，我们开发了三种新的qPCR检测方法。对每个亚种的个体和两个非目标物种，即圆腹蛛和圆腹蛛进行了分析验证。一种测定法仅扩增P.australis亚种。美洲，一个扩增了P.australis亚种。australis和/或P.australis亚种。berlandieri，其中一个扩增了P.australis亚种。americanus和/或P.australis亚种。澳大利亚。该方案通过提供所有三个亚种的遗传歧视，增强了目前可用的快速鉴定方法。结论使用来自美国各地的P.australis样品对新开发的测定方法进行了验证。在该地理范围之外应用这些分析之前，应进行额外的测试。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464