Dynamic interactions between Candida albicans and different streptococcal species in a multispecies oral biofilm

IF 3.9 3区 生物学 Q2 MICROBIOLOGY
MicrobiologyOpen Pub Date : 2023-10-03 DOI:10.1002/mbo3.1381
Tenzin Kunchok Lueyar, Lamprini Karygianni, Thomas Attin, Thomas Thurnheer
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Abstract

The oral cavity is colonized by a plethora of bacteria, fungi, and archaea, including streptococci of the mitis group (MSG) and the yeast Candida albicans. This study aims to investigate the role of streptococcal species in the development of oral biofilm and the cross-kingdom interactions between some of the members of the commensal MSG and the pathogen yeast C. albicans using a multispecies supragingival biofilm model. A total of nine different in vitro biofilms were grown, quantified with culture analyses, and visually examined with confocal laser scanning microscopy (CLSM). A four-species biofilm without any streptococcal species was used as a basic biofilm. In each subsequent inoculum, one species of MSG was added and afterward combined with Streptococcus mutans. The eight-species biofilm contained all eight strains used in this study. Culture analyses showed that the presence of S. mutans in a four-species biofilm with Streptococcus oralis or S. oralis subsp. tigurinus did not differ significantly in C. albicans colony-forming unit (CFU) counts compared to biofilms without S. mutans. However, compared to other mitis species, Streptococcus gordonii combined with S. mutans resulted in the lowest CFUs of C. albicans. Visual observation by CLSM showed that biofilms containing both S. mutans and one species of MSG seemed to induce the formation of filamentous form of C. albicans. However, when several species of MSG were combined with S. mutans, C. albicans was again found in its yeast form.

Abstract Image

多菌种口腔生物膜中白色念珠菌与不同链球菌的动态相互作用
口腔被大量细菌、真菌和古菌定植,包括轻度炎症链球菌(MSG)和白色念珠菌。本研究旨在使用多物种牙龈上生物膜模型研究链球菌在口腔生物膜形成中的作用,以及共生MSG的一些成员与病原体白色念珠菌之间的跨王国相互作用。共生长了九种不同的体外生物膜,通过培养分析进行定量,并通过共聚焦激光扫描显微镜(CLSM)进行目视检查。使用不含任何链球菌的四种生物膜作为基本生物膜。在随后的每种接种物中,加入一种MSG,然后与变形链球菌结合。八种生物膜包含本研究中使用的所有八种菌株。培养分析表明,口腔链球菌或口腔链球菌亚种的四种生物膜中存在变异链球菌。与没有变异链球菌的生物膜相比,tigurinus在白色念珠菌菌落形成单位(CFU)计数方面没有显著差异。然而,与其他mitis物种相比,戈登链球菌与变形链球菌联合产生的白色念珠菌CFU最低。CLSM的视觉观察表明,含有变形链球菌和一种MSG的生物膜似乎能诱导白色念珠菌的丝状形成。然而,当几种MSG与变形链球菌结合时,白色念珠菌再次以酵母形式被发现。
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来源期刊
MicrobiologyOpen
MicrobiologyOpen MICROBIOLOGY-
CiteScore
8.00
自引率
0.00%
发文量
78
审稿时长
20 weeks
期刊介绍: MicrobiologyOpen is a peer reviewed, fully open access, broad-scope, and interdisciplinary journal delivering rapid decisions and fast publication of microbial science, a field which is undergoing a profound and exciting evolution in this post-genomic era. The journal aims to serve the research community by providing a vehicle for authors wishing to publish quality research in both fundamental and applied microbiology. Our goal is to publish articles that stimulate discussion and debate, as well as add to our knowledge base and further the understanding of microbial interactions and microbial processes. MicrobiologyOpen gives prompt and equal consideration to articles reporting theoretical, experimental, applied, and descriptive work in all aspects of bacteriology, virology, mycology and protistology, including, but not limited to: - agriculture - antimicrobial resistance - astrobiology - biochemistry - biotechnology - cell and molecular biology - clinical microbiology - computational, systems, and synthetic microbiology - environmental science - evolutionary biology, ecology, and systematics - food science and technology - genetics and genomics - geobiology and earth science - host-microbe interactions - infectious diseases - natural products discovery - pharmaceutical and medicinal chemistry - physiology - plant pathology - veterinary microbiology We will consider submissions across unicellular and cell-cluster organisms: prokaryotes (bacteria, archaea) and eukaryotes (fungi, protists, microalgae, lichens), as well as viruses and prions infecting or interacting with microorganisms, plants and animals, including genetic, biochemical, biophysical, bioinformatic and structural analyses. The journal features Original Articles (including full Research articles, Method articles, and Short Communications), Commentaries, Reviews, and Editorials. Original papers must report well-conducted research with conclusions supported by the data presented in the article. We also support confirmatory research and aim to work with authors to meet reviewer expectations. MicrobiologyOpen publishes articles submitted directly to the journal and those referred from other Wiley journals.
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