Mast cells in goldfish (Carassius auratus) gut: Immunohistochemical characterization

IF 1.1 4区生物学 Q4 ANATOMY & MORPHOLOGY

Acta Zoologica Pub Date : 2022-03-01 DOI:10.1111/azo.12417

Alessio Alesci, Simona Pergolizzi, Angelo Fumia, Concetta Calabrò, Patrizia Lo Cascio, Eugenia Rita Lauriano

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引用次数: 29

Abstract

The common goldfish is the most widespread teleosts in the world. Due to its peculiar characteristics, such as the high resistance, easy availability and stabulation, and for its evolutionary characteristics, this fish lends itself to be one of the most used experimental models. This study aimed to characterize the mast cells in the intestine of Carassius auratus using anti-TLR-2, anti-S100, anti-VIP, anti-serotonin (5-HT) and anti-Piscidin antibodies. The intestine of goldfish, like that of all vertebrates, plays an important role in the immunology of the animal. The gut-associated lymphoid tissue GALT is an immune component containing several specific cells such as lymphocytes, macrophages and mast cells. In addition, the presence of goblet cells in the intestinal epithelium strengthens the defence system, secreting many cytokines and chemokines and displaying antibacterial properties. Our results show mast cells labelled with antibodies that are highly conserved between fish and mammals, demonstrating an active role of these cells in the immune response.

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金鱼（Carassius auratus）肠道肥大细胞的免疫组织化学特征

常见的金鱼是世界上分布最广的硬骨鱼。由于其独特的特性，如高阻力、易于获得和稳定，以及其进化特性，这种鱼成为最常用的实验模型之一。本研究旨在利用抗TLR-2、抗S100、抗VIP、抗5-羟色胺（5-HT）和抗Piscidin抗体对鲫鱼肠道肥大细胞进行鉴定。金鱼的肠道和所有脊椎动物的肠道一样，在动物的免疫学中起着重要作用。肠道相关淋巴组织GALT是一种免疫成分，包含几种特定细胞，如淋巴细胞、巨噬细胞和肥大细胞。此外，肠上皮中杯状细胞的存在增强了防御系统，分泌许多细胞因子和趋化因子，并显示出抗菌特性。我们的研究结果显示，用抗体标记的肥大细胞在鱼类和哺乳动物之间高度保守，证明了这些细胞在免疫反应中的积极作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Zoologica 生物-动物学

CiteScore

2.90

自引率

8.30%

发文量

审稿时长

>12 weeks

期刊介绍： Published regularly since 1920, Acta Zoologica has retained its position as one of the world''s leading journals in the field of animal organization, development, structure and function. Each issue publishes original research of interest to zoologists and physiologists worldwide, in the field of animal structure (from the cellular to the organismic level) and development with emphasis on functional, comparative and phylogenetic aspects. Occasional review articles are also published, as well as book reviews.