Characterization of meiotic chromosome behavior in the autopolyploid Saccharum spontaneum reveals preferential chromosome pairing without distinct DNA sequence variation

IF 6 1区 农林科学 Q1 AGRONOMY
Xin Zhang , Zhuang Meng , Jinlei Han , Haris Khurshid , Ayman Esh , Robert Hasterok , Kai Wang
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引用次数: 1

Abstract

Autopolyploidy and allopolyploidy may represent an evolutionary advantage and are more common in plants than assumed. However, less attention has been paid to autopolyploidy than to allopolyploidy, and its evolutionary consequences are largely unclear, especially for plants with high ploidy levels. In this study, we developed oligonucleotide (oligo)-based chromosome painting probes to identify individual chromosomes in S. spontaneum. Using fluorescence in situ hybridization (FISH), we investigated chromosome behavior during pachytene, metaphase, anaphase, and telophase of meiosis I (MI) in autotetraploid, autooctoploid, and autodecaploid S. spontaneum clones. All autopolyploid clones showed stable diploidized chromosome behavior; so that homologous chromosomes formed almost exclusively bivalents during MI. Two copies of homologous chromosome 8 with similar sizes in the autotetraploid clone showed preferential pairing with each other with respect to the other copies. However, sequence variation analysis showed no apparent differences among homologs of chromosome 8 and all other chromosomes. We suggest that either the stable diploidized pairing or the preferential pairing between homologous copies of chromosome 8 in the studied autopolyploid sugarcane are accounted for by unknown mechanisms other than DNA sequence similarity. Our results reveal evolutionary consequences of stable meiotic behavior in autopolyploid plants.

自多倍体糖的减数分裂染色体行为特征揭示了没有明显DNA序列变异的优先染色体配对
自多倍体和异源多倍体可能代表了一种进化优势,并且在植物中比假设的更常见。然而,与异源多倍体相比,对自多倍体的关注较少,其进化结果在很大程度上是不清楚的,特别是对于具有高倍性水平的植物。在这项研究中,我们开发了基于寡核苷酸(oligo)的染色体涂绘探针来鉴定天然稻的单个染色体。利用荧光原位杂交(FISH)技术,研究了自四倍体、自十倍体和自十倍体自发性海百合(S. spontanum)克隆减数分裂I (MI)的粗期、中期、后期和末期的染色体行为。所有自多倍体克隆均表现出稳定的染色体二倍体化行为;同源染色体几乎完全形成二价体。同源8号染色体在同源四倍体克隆中具有相似大小的两个拷贝相对于其他拷贝表现出优先配对。序列变异分析显示,8号染色体与其他所有染色体的同源物间无明显差异。我们认为,在研究的自多倍体甘蔗中,8号染色体同源拷贝之间的稳定二倍体配对或优先配对可能是由DNA序列相似性以外的未知机制造成的。我们的研究结果揭示了自多倍体植物稳定减数分裂行为的进化后果。
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来源期刊
Crop Journal
Crop Journal Agricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
9.90
自引率
3.00%
发文量
638
审稿时长
41 days
期刊介绍: The major aims of The Crop Journal are to report recent progresses in crop sciences including crop genetics, breeding, agronomy, crop physiology, germplasm resources, grain chemistry, grain storage and processing, crop management practices, crop biotechnology, and biomathematics. The regular columns of the journal are Original Research Articles, Reviews, and Research Notes. The strict peer-review procedure will guarantee the academic level and raise the reputation of the journal. The readership of the journal is for crop science researchers, students of agricultural colleges and universities, and persons with similar academic levels.
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