Miranda Torre , Sara M. Giannitelli , Emanuele Mauri , Manuele Gori , Alessio Bucciarelli , Pamela Mozetic , Giuseppe Gigli , Marcella Trombetta , Alberto Rainer
{"title":"Printability assessment workflow of a thermosensitive photocurable biomaterial ink for microextrusion bioprinting","authors":"Miranda Torre , Sara M. Giannitelli , Emanuele Mauri , Manuele Gori , Alessio Bucciarelli , Pamela Mozetic , Giuseppe Gigli , Marcella Trombetta , Alberto Rainer","doi":"10.1016/j.bprint.2023.e00262","DOIUrl":null,"url":null,"abstract":"<div><p>Microextrusion bioprinting enables heterogeneous constructs with high shape fidelity to be fabricated through the deposition of a bioink with the desired physico-chemical and biological characteristics.</p><p>In this work, a novel semi-synthetic hydrogel, consisting of gelatin methacrylate<span><span> and Pluronic F127, has been specifically formulated to match the requirements of microextrusion bioprinting process. By merging the thermosensitive characteristics of Pluronic with the cross-linking features of gelatin methacrylate, the formulation showed a printability window characterized by good shape retention and chemical stability following photo-crosslinking, as demonstrated by a thorough printability assessment, performed employing empirical predictive models. The </span>mechanical properties<span><span> of the constructs were comparable to those of soft tissues, widening the range of applicability in soft tissue engineering<span>. The bioink was successfully applied to the fabrication of multilayered porous constructs preserving high levels of cell viability. Interestingly, the spatial arrangement of the cells showed a high degree of alignment along the deposition direction. Overall, the </span></span>manufacturing process developed herein could represent a promising strategy to design three-dimensional models with predetermined cellular alignment.</span></span></p></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioprinting","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2405886623000052","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Computer Science","Score":null,"Total":0}
引用次数: 1
Abstract
Microextrusion bioprinting enables heterogeneous constructs with high shape fidelity to be fabricated through the deposition of a bioink with the desired physico-chemical and biological characteristics.
In this work, a novel semi-synthetic hydrogel, consisting of gelatin methacrylate and Pluronic F127, has been specifically formulated to match the requirements of microextrusion bioprinting process. By merging the thermosensitive characteristics of Pluronic with the cross-linking features of gelatin methacrylate, the formulation showed a printability window characterized by good shape retention and chemical stability following photo-crosslinking, as demonstrated by a thorough printability assessment, performed employing empirical predictive models. The mechanical properties of the constructs were comparable to those of soft tissues, widening the range of applicability in soft tissue engineering. The bioink was successfully applied to the fabrication of multilayered porous constructs preserving high levels of cell viability. Interestingly, the spatial arrangement of the cells showed a high degree of alignment along the deposition direction. Overall, the manufacturing process developed herein could represent a promising strategy to design three-dimensional models with predetermined cellular alignment.
期刊介绍:
Bioprinting is a broad-spectrum, multidisciplinary journal that covers all aspects of 3D fabrication technology involving biological tissues, organs and cells for medical and biotechnology applications. Topics covered include nanomaterials, biomaterials, scaffolds, 3D printing technology, imaging and CAD/CAM software and hardware, post-printing bioreactor maturation, cell and biological factor patterning, biofabrication, tissue engineering and other applications of 3D bioprinting technology. Bioprinting publishes research reports describing novel results with high clinical significance in all areas of 3D bioprinting research. Bioprinting issues contain a wide variety of review and analysis articles covering topics relevant to 3D bioprinting ranging from basic biological, material and technical advances to pre-clinical and clinical applications of 3D bioprinting.