Characterization and genomic analysis of a novel Pseudoalteromonas phage PS_L5

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-09-14 DOI:10.1016/j.margen.2023.101069

Xing Zhou , Jie Gao , Rongda Xiao , Yifan Qiao , Yuang Zhu , Di Zhang , Xinyu Zhang , Huifang Li , Juntian Xu

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引用次数: 0

Abstract

Pseudoalteromonas is a widely distributed bacterial genus that is associated with marine algae. However, there is still limited knowledge about their bacteriophage. In this study, we reported the isolation of a novel lytic bacteriophage that infects Pseudoalteromonas marina. Transmission electron microscopy revealed that PS_L5 had an icosahedral head of 52.6 ± 2 nm and a non-contractile tail with length of 96.5 ± 2 nm. The genome sequence of this phage was 34, 257 bp and had a GC content of 40.75%. Furthermore, this genome contained 61 predicted open reading frames (ORFs), which involved in various functions such as phage structure, packaging, DNA metabolism, host lysis and other additional functions. Additionally, the phylogenetic analysis based on major capsid protein showed that the phage PS_L5 was closely related to five other Pseudoalteromonas phages, namely PHS3, PHS21, AL, SL25 and Pq0 which also possessed the non-contractile long tail. This study provided the fundamental insights into the evolutionary dynamics of Pseudoalteromonas phages and the interaction between phage and host.

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假互交单胞菌噬菌体PS_L5的鉴定与基因组分析

假交替单胞菌是一个分布广泛的细菌属，与海藻有关。然而，对它们的噬菌体的了解仍然有限。在这项研究中，我们报道了一种新的裂解噬菌体的分离，它感染了码头假交替单胞菌。透射电子显微镜显示，PS_L5具有52.6±2nm的二十面体头部和96.5±2nm长的不收缩尾部。该噬菌体的基因组序列为35257bp，GC含量为40.75%。此外，该基因组包含61个预测开放阅读框（ORF），它们涉及噬菌体的结构、包装、DNA代谢等多种功能，宿主裂解和其他附加功能。此外，基于主要衣壳蛋白的系统发育分析表明，噬菌体PS_L5与其他五种Pseudoalteromonas噬菌体密切相关，即PHS3、PHS21、AL、SL25和Pq0，它们也具有不可收缩的长尾。本研究为假交替单胞菌噬菌体的进化动力学以及噬菌体与宿主之间的相互作用提供了基本的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.