Complete genome sequence of Lactobacillus fermentum 9-4, a purine-degrading Lactobacillus probiotic isolated from Chinese fermented rice-flour noodles

IF 5.2 Q1 FOOD SCIENCE & TECHNOLOGY
Tiantian Gao, Lianghua Lu, Qingling Wu, Chenghua Wang
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引用次数: 1

Abstract

Lactobacillus fermentum 9-4 is a purine-degrading probiotic firstly isolated from traditional fermented rice-flour noodles with special characteristics of southern China's Guangxi Zhuang Autonomous Region. L. fermentum 9-4 could efficiently reduce the purine contents in foods with excellent purine-nucleoside assimilation and purine-metabolizing enzymatic activities. Crude cell lysate of L. fermentum 9-4 showed strong purine degradation abilities on nucleosides including inosine, guanosine, and purines including xanthine and uric acid, indicating a complete purine metabolic pathway to degrade purine substances to allantoin. In this study, the whole genome of L. fermentum 9-4 was sequenced. Its genome consisted of a 2 085 632 bp circular chromosome without plasmid. The GC content of circular chromosome was 51.25%, including 2 089 coding sequences (CDs), 15 rRNA operons and 59 tRNA coding genes. Genetic analysis showed that at least 5 protein coding genes were related to purine lowering ability. In addition, neither toxic virulence factor nor drug resistance gene was identified by searching the virulence factor database and drug resistance gene database, suggesting good safety of L. fermentum 9-4. The results showed that L. fermentum 9-4 has a good potential for the development and utilization as a purine-degrading probiotic for low-purine foods.

从发酵米粉中分离的嘌呤降解益生乳杆菌发酵乳杆菌9-4的全基因组序列
发酵乳杆菌9-4是从广西壮族自治区传统发酵米粉中首次分离得到的一种嘌呤降解益生菌。发酵乳杆菌9-4具有良好的嘌呤核苷同化和嘌呤代谢酶活性,可有效降低食品中的嘌呤含量。发酵乳杆菌9-4的粗细胞裂解物对核苷(包括肌苷、鸟苷)和嘌呤(包括黄嘌呤和尿酸)表现出较强的嘌呤降解能力,表明嘌呤物质降解为尿囊素的完整嘌呤代谢途径。本研究对发酵乳杆菌9-4的全基因组进行了测序。其基因组由2 085 632 bp的环状染色体组成,无质粒。环状染色体GC含量为51.25%,包括2089个编码序列、15个rRNA操纵子和59个tRNA编码基因。遗传分析表明,至少有5个蛋白质编码基因与嘌呤降低能力有关。此外,通过检索毒力因子数据库和耐药性基因数据库,未鉴定出毒性毒力因子和耐药性基因,表明发酵乳杆菌9-4具有良好的安全性。结果表明,发酵乳杆菌9-4作为一种用于低嘌呤食品的嘌呤降解益生菌具有良好的开发利用潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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CiteScore
5.80
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0.00%
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