GmNLP7a inhibits soybean nodulation by interacting with GmNIN1a

IF 6 1区农林科学 Q1 AGRONOMY

Crop Journal Pub Date : 2023-10-01 DOI:10.1016/j.cj.2023.03.016

Xuesong Wu , Yuping Xiong , Jingjing Lu, Mi Yang, Hongtao Ji, Xia Li, Zhijuan Wang

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引用次数: 0

Abstract

Nitrogen (N) is an essential macronutrient for plant growth and productivity. Leguminous plants establish symbiotic relationships with nitrogen-fixing rhizobial bacteria to use atmospheric dinitrogen gas to meet high N demand under low-N conditions. Nodule formation and N fixation are energy-consuming processes and are inhibited by nitrate present in the environment. Previous studies in model leguminous plants characterized NIN-LIKE PROTEIN (NLP) proteins that mediate nitrate control of root nodule symbiosis, but the mechanism by which nitrate regulates soybean root nodules via NLP remains unclear. In the soybean genome we found four homologs of AtNLP7, named GmNLP7a–GmNLP7d. We showed that the expression of GmNLP7s is responsive to nitrate but not to rhizobial infection and localized GmNLP7a to the nucleus. Downregulation of GmNLP7s increased nodule number, and overexpression of GmNLP7a (GmNLP7aOE) reduced nodule number regardless of nitrate availability, suggesting a negative role for GmNLP7s in nodulation. Nitrogenase activity in the GmNLP7aOE line was comparable to that of the wild type, indicating that GmNLP7a does not affect mature nodule activity. Overexpression of GmNLP7a downregulated the expression of GmNIN1a and GmENOD40-1. GmNLP7a interacted with GmNIN1a via the PB1 domain. Our results reveal a new regulator of GmNLP7 in nodulation and a molecular mechanism by which nitrate affects nodule number in soybean.

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GmNLP7a通过与GmNIN1a相互作用抑制大豆结瘤

氮（N）是植物生长和生产力所必需的大量营养素。豆科植物与固氮根瘤菌建立共生关系，利用大气中的二氮气体在低氮条件下满足高氮需求。根瘤的形成和氮的固定是耗能过程，并受到环境中存在的硝酸盐的抑制。先前在模型豆科植物中的研究以介导硝酸盐控制根瘤共生的九类蛋白（NLP）为特征，但硝酸盐通过NLP调节大豆根瘤的机制尚不清楚。在大豆基因组中，我们发现了AtNLP7的四个同源物，命名为GmNLP7a–GmNLP7d。我们发现GmNLP7s的表达对硝酸盐有反应，但对根瘤菌感染没有反应，并将GmNLP7a定位于细胞核。GmNLP7s的下调增加了结节数量，而无论硝酸盐的可用性如何，GmNLP7a的过表达都减少了结节数量。GmNLP7aOE系中的固氮酶活性与野生型相当，表明GmNLP7a不影响成熟根瘤的活性。GmNLP7a的过表达下调了GmNIN1a和GmENOD40-1的表达。GmNLP7a通过PB1结构域与GmNIN1a相互作用。我们的研究结果揭示了GmNLP7在结瘤中的一种新的调节因子，以及硝酸盐影响大豆结瘤数量的分子机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Crop Journal Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

9.90

自引率

3.00%

发文量

638

审稿时长

41 days

期刊介绍： The major aims of The Crop Journal are to report recent progresses in crop sciences including crop genetics, breeding, agronomy, crop physiology, germplasm resources, grain chemistry, grain storage and processing, crop management practices, crop biotechnology, and biomathematics. The regular columns of the journal are Original Research Articles, Reviews, and Research Notes. The strict peer-review procedure will guarantee the academic level and raise the reputation of the journal. The readership of the journal is for crop science researchers, students of agricultural colleges and universities, and persons with similar academic levels.