Iron Toxicity Tolerance of Rice Genotypes in Relation to Growth, Yield and Physiochemical Characters

Abstract

Iron (Fe) toxicity, generated from excess reduced ferrous Fe (Fe²⁺) ion formation within the soil under submerged condition, is a potent environmental stress that limits lowland rice production. Total 11 diverse Thai rice genotypes, including a recognized tolerant genotype Azucena and a susceptible genotype IR64, were evaluated against 5 Fe²⁺ levels [0 (control), 150, 300, 600 and 900 mg/L] to screen the tested genotypes for their Fe-toxicity tolerance and to classify them as a sensitive/tolerant category. The evaluation was conducted by a germination study, followed by a polyhouse study on growth, yield and physiochemical performances. Results showed significant variations in Fe²⁺-tolerance across genotypes. Increasing Fe²⁺ level beyond 300 mg/L was detrimental for germination and growth of all the tested genotypes, although germination responses were negatively affected at Fe²⁺ ≥ 300 mg/L. Physiochemical responses in the form of leaf greenness, net photosynthetic rate, membrane stability index and Fe contents in leaf and root were the most representative of Fe²⁺-toxicity-mediated impairments on overall growth and yield. Difference in physiochemical responses was effectively correlated with the contrasting ability of the genotypes on lowering excess Fe²⁺ in tissues. Analysis of average tolerance and stress tolerance index unveiled that the genotypes RD85 and RD31 were the closest to the tolerant check Azucena and the sensitive check IR64, respectively. The unweighted pair group method with arithmetic means clustering revealed three major clusters, with cluster II (four genotypes) being Fe²⁺ tolerant and cluster I (four genotypes) being Fe²⁺ sensitive. Principal component (PC) analysis and genotype by trait-biplot analysis showed that the first two components explained 90.5% of the total variation, with PC1 accounting for 56.6% and PC2 for 33.9% of the total variation. The identified tolerant rice genotypes show potentials for cultivation in Fe²⁺-toxic lowlands for increased productivity. The findings contribute to the present understanding on Fe²⁺-toxicity response and provide a basis for future genotype selection or rice crop improvement programs against Fe²⁺-toxicity.