A cluster of mutagenesis revealed an osmotic regulatory role of the OsPIP1 genes in enhancing rice salt tolerance

IF 6 1区 农林科学 Q1 AGRONOMY
Leyuan Tao , Bing Wang , Shichao Xin , Wei Li , Shengcai Huang , Laihua Liu , Jing Cui , Qianru Zhang , Xianguo Cheng
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引用次数: 1

Abstract

Aquaporins play important regulatory roles in improving plant abiotic stress tolerance. To better understand whether the OsPIP1 genes collectively dominate the osmotic regulation in rice under salt stress, a cluster editing of the OsPIP1;1, OsPIP1;2 and OsPIP1;3 genes in rice was performed by CRISPR/Cas9 system. Sequencing showed that two mutants with Cas9-free, line 14 and line 18 were successfully edited. Briefly, line 14 deleted a single C base in both the OsPIP1;1 and OsPIP1;3 genes, and inserted a single T base in the OsPIP1;2 gene, respectively. While line 18 demonstrated an insertion of a single A base in the OsPIP1;1 gene and a single T base in both the OsPIP1;2 and OsPIP1;3 genes, respectively. Multiplex editing of the OsPIP1 genes significantly inhibited photosynthetic rate and accumulation of compatible metabolites, but increased MDA contents and osmotic potentials in the mutants, thus delaying rice growth under salt stress. Functional loss of the OsPIP1 genes obviously suppressed the expressions of the OsPIP1, OsSOS1, OsCIPK24 and OsCBL4 genes, and increased the influxes of Na+ and effluxes of K+/H+ in the roots, thus accumulating more Na+ in rice mutants under salt stress. This study suggests that the OsPIP1 genes are essential modulators collectively contributing to the enhancement of rice salt stress tolerance, and multiplex editing of the OsPIP1 genes provides insight into the osmotic regulation of the PIP genes.

一组突变揭示了OsPIP1基因在提高水稻耐盐性中的渗透调节作用
水通道蛋白在提高植物非生物胁迫耐受性方面发挥着重要的调控作用。为了更好地了解OsPIP1基因是否共同主导盐胁迫下水稻的渗透调节,对OsPIP1进行了聚类编辑;1、OsPIP1;2和OsPIP1;利用CRISPR/Cas9系统对水稻中的3个基因进行了测序。测序结果表明,成功编辑了两个不含Cas9的突变体,系14和系18。简言之,第14行删除了两个OsPIP1中的单个C碱基;1和OsPIP1;3个基因,并在OsPIP1中插入单个T碱基;2基因。而线18展示了在OsPIP1中插入单个a碱基;1基因和两个OsPIP1中的单个T碱基;2和OsPIP1;3个基因。OsPIP1基因的多重编辑显著抑制了突变体的光合速率和相容代谢产物的积累,但增加了突变体中的MDA含量和渗透电位,从而延缓了盐胁迫下水稻的生长。OsPIP1基因的功能缺失明显抑制了OsPIP1、OsSOS1、OsCIPK24和OsCBL4基因的表达,并增加了Na+在根中的流入和K+/H+的流出,从而在盐胁迫下积累了更多的Na+。这项研究表明,OsPIP1基因是共同促进水稻耐盐性增强的重要调节剂,对OsPIP1的多重编辑提供了对PIP基因渗透调节的深入了解。
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来源期刊
Crop Journal
Crop Journal Agricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
9.90
自引率
3.00%
发文量
638
审稿时长
41 days
期刊介绍: The major aims of The Crop Journal are to report recent progresses in crop sciences including crop genetics, breeding, agronomy, crop physiology, germplasm resources, grain chemistry, grain storage and processing, crop management practices, crop biotechnology, and biomathematics. The regular columns of the journal are Original Research Articles, Reviews, and Research Notes. The strict peer-review procedure will guarantee the academic level and raise the reputation of the journal. The readership of the journal is for crop science researchers, students of agricultural colleges and universities, and persons with similar academic levels.
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