Identification of stable quantitative trait loci underlying waterlogging tolerance post-anthesis in common wheat (Triticum aestivum)

IF 6 1区 农林科学 Q1 AGRONOMY
Fugong Ding , Jingyang Tong , Rui Xu , Jing Chen , Xiaoting Xu , Muhammad Nadeem , Shuping Wang , Yingxin Zhang , Zhanwang Zhu , Fengju Wang , Zhengwu Fang , Yuanfeng Hao
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引用次数: 1

Abstract

Waterlogging is a growing threat to wheat production in high-rainfall areas. In this study, a doubled haploid (DH) population developed from a cross between Yangmai 16 (waterlogging-tolerant) and Zhongmai 895 (waterlogging-sensitive) was used to map quantitative trait loci (QTL) for waterlogging tolerance using a high-density 660K single-nucleotide polymorphism (SNP) array. Two experimental designs, waterlogging concrete tank (CT) and waterlogging plastic tank (PT), were used to simulate waterlogging during anthesis in five environments across three growing seasons. Waterlogging significantly decreased thousand-kernel weight (TKW) relative to non-waterlogged controls, although the degree varied across lines. Three QTL for waterlogging tolerance were identified on chromosomes 4AL, 5AS, and 7DL in at least two environments. All favorable alleles were contributed by the waterlogging-tolerant parent Yangmai 16. QWTC.caas-4AL exhibited pleiotropic effects on both enhancing waterlogging tolerance and decreasing plant height. Six high-confidence genes were annotated within the QTL interval. The combined effects of QWTC.caas-4AL and QWTC.caas-5AS greatly improved waterlogging tolerance, while the combined effects of all three identified QTL (QWTC.caas-4AL, QWTC.caas-5AS, and QWTC.caas-7DL) exhibited the most significant effect on waterlogging tolerance. Breeder-friendly kompetitive allele-specific PCR (KASP) markers (K_AX_111523809, K_AX_108971224, and K_AX_110553316) flanking the interval of QWTC.caas-4AL, QWTC.caas-5AS, and QWTC.caas-7DL were produced. These markers were tested in a collection of 240 wheat accessions, and three superior polymorphisms of the markers distributed over 67 elite cultivars in the test population, from the Chinese provinces of Jiangsu, Anhui, and Hubei. The three KASP markers could be used for marker-assisted selection (MAS) to improve waterlogging tolerance in wheat.

普通小麦花后耐涝性稳定数量性状基因座的鉴定
在高降雨量地区,水涝对小麦生产的威胁越来越大。本研究采用高密度660K单核苷酸多态性(SNP)阵列,以杨麦16号(耐涝)和中麦895号(耐渍)杂交形成的双单倍体(DH)群体为材料,定位了耐涝性的数量性状位点(QTL)。采用混凝土内涝池和塑料内涝池两种实验设计,在三个生长季节的五个环境中模拟了花期内涝。与非淹水对照相比,淹水显著降低了千粒重(TKW),尽管不同品系的程度不同。在至少两个环境中,在染色体4AL、5AS和7DL上鉴定出三个耐涝QTL。所有有利的等位基因均由耐涝亲本扬麦16贡献。QWTC.caas-4AL具有提高耐涝性和降低株高的多效性作用。在QTL区间内注释了6个高置信度基因。QWTC.caas-4AL和QWTC.caas-5AS的联合作用大大提高了耐涝性,而三个已鉴定的QTL(QWTC.caas-4AL、QWTC.caas-5AS和QWTC.caas-7DL)的联合作用对耐涝性表现出最显著的影响。产生了位于QWTC.caas-4AL、QWTC.caas-5AS和QWTC.cab as-7DL区间两侧的对育种者友好的竞争性等位基因特异性PCR(KASP)标记(K_AX_111523809、K_AX_108971224和K_AX_110553316)。这些标记在240份小麦材料中进行了测试,这些标记的三个优势多态性分布在来自中国江苏、安徽和湖北省的67个优良品种上。三个KASP标记可用于标记辅助选择(MAS),以提高小麦的耐涝性。
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来源期刊
Crop Journal
Crop Journal Agricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
9.90
自引率
3.00%
发文量
638
审稿时长
41 days
期刊介绍: The major aims of The Crop Journal are to report recent progresses in crop sciences including crop genetics, breeding, agronomy, crop physiology, germplasm resources, grain chemistry, grain storage and processing, crop management practices, crop biotechnology, and biomathematics. The regular columns of the journal are Original Research Articles, Reviews, and Research Notes. The strict peer-review procedure will guarantee the academic level and raise the reputation of the journal. The readership of the journal is for crop science researchers, students of agricultural colleges and universities, and persons with similar academic levels.
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