The pentatricopeptide repeat protein EMP601 functions in maize seed development by affecting RNA editing of mitochondrial transcript ccmC

IF 6 1区 农林科学 Q1 AGRONOMY
Rongrong Chen , Qianhan Wei , Yan Liu , Jiankun Li , Xuemei Du , Yan Chen , Jianhua Wang , Yunjun Liu
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引用次数: 1

Abstract

Although several pentatricopeptide repeat (PPR) proteins are involved in post-transcriptional processing of mitochondrial RNA, it is unclear which specific protein is involved in the RNA editing of ccmC in maize (Zea mays). Here we report the identification of the maize empty pericarp 601 (emp601) mutant and the map-based cloning of the Emp601 gene, which encodes an E2-type PPR protein that is targeted to mitochondria. A single-nucleotide deletion in the emp601 mutant caused a frameshift and introduced a premature stop codon into the predicted EMP601. This mutation was associated with reduced accumulation of mitochondrial complex III as well as with inhibition of growth and differentiation of basal endosperm transfer layer cells, leading to final degeneration of the embryo and endosperm. We determine that loss of EMP601 function prevents the C-to-U RNA editing of the mitochondrial transcript ccmC at position 358. EMP601 binds to the ccmC transcript and directly interacts with Multiple organellar RNA editing factor 8 and may be a component of the plant mitochondrial editosome. We conclude that EMP601 functions in RNA editing of mitochondrial ccmC transcripts and influences mitochondrial function and seed development.

五肽重复序列蛋白EMP601通过影响线粒体转录物ccmC的RNA编辑在玉米种子发育中的作用
尽管几种五肽重复序列(PPR)蛋白参与线粒体RNA的转录后处理,但尚不清楚哪种特定蛋白参与玉米ccmC的RNA编辑。在此,我们报道了玉米空果皮601(emp601)突变体的鉴定和基于图谱的emp601基因的克隆,该基因编码一种靶向线粒体的E2型PPR蛋白。emp601突变体中的单核苷酸缺失引起移码,并将过早终止密码子引入预测的emp601中。这种突变与线粒体复合体III的积累减少以及基底胚乳转移层细胞的生长和分化受到抑制有关,导致胚胎和胚乳的最终退化。我们确定EMP601功能的丧失阻止了线粒体转录物ccmC在358位的C-to-U RNA编辑。EMP601与ccmC转录物结合并直接与多器官RNA编辑因子8相互作用,并且可能是植物线粒体编辑体的组成部分。我们得出结论,EMP601在线粒体ccmC转录物的RNA编辑中发挥作用,并影响线粒体功能和种子发育。
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来源期刊
Crop Journal
Crop Journal Agricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
9.90
自引率
3.00%
发文量
638
审稿时长
41 days
期刊介绍: The major aims of The Crop Journal are to report recent progresses in crop sciences including crop genetics, breeding, agronomy, crop physiology, germplasm resources, grain chemistry, grain storage and processing, crop management practices, crop biotechnology, and biomathematics. The regular columns of the journal are Original Research Articles, Reviews, and Research Notes. The strict peer-review procedure will guarantee the academic level and raise the reputation of the journal. The readership of the journal is for crop science researchers, students of agricultural colleges and universities, and persons with similar academic levels.
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