Introgression of sharp eyespot resistance from Dasypyrum villosum chromosome 2VL into bread wheat

IF 6 1区农林科学 Q1 AGRONOMY

Crop Journal Pub Date : 2023-10-01 DOI:10.1016/j.cj.2023.04.013

Caiyun Liu , Wei Guo , Yang Wang , Bisheng Fu , Jaroslav Doležel , Ying Liu , Wenling Zhai , Mahmoud Said , István Molnár , Kateřina Holušová , Ruiqi Zhang , Jizhong Wu

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引用次数: 2

Abstract

Wheat sharp eyespot, a stem disease caused by the soilborne fungus Rhizoctonia cerealis van der Hoeven, has become a threat to wheat production worldwide. Exploiting resistance resources from wild relatives of wheat is a promising strategy for controlling this disease. In this study, a new wheat–Dasypyrum villosum T2DS·2V#4L translocation line in the background of Chinese Spring (CS) showed stable resistance to R. cerealis. Introgression of the T2DS·2V#4L chromosome into wheat cultivar Aikang 58 by backcrossing produced a marked increase in sharp eyespot resistance in NIL-T2DS·2V#4L in comparison with NIL-T2DS·2DL, and no detrimental effects of 2V#4L on agronomic traits were observed in the BC₂F₂, BC₂F_2:3, and BC₂F_2:4 generations. Flow-sorted sequencing of 2V#4L yielded 384.3 Mb of assembled sequence, and 8836 genes were predicted of which 6154 had orthologs in at least one of the 2AL, 2BL, and 2DL arms of CS, whereas 1549 genes were unique to 2V#4L. About 100,000 SNPs were detected in genes of 2V#4L and 2DL in 10 sequenced bread wheat cultivars. A Kompetitive Allele Specific Polymerase chain reaction and 30 conserved ortholog sequence markers were developed to trace the 2V#4L chromatin in wheat backgrounds. T2DS·2V#4L compensating translocation lines represent novel germplasm with sharp eyespot resistance and the markers will allow rapid detection in breeding programs.

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绒毛草2VL染色体对小麦尖斑病抗性的导入

小麦尖叶病是一种由土壤真菌蜡状丝核菌引起的茎部疾病，已对全球小麦生产构成威胁。利用小麦野生近缘品种的抗性资源是防治该病的一个很有前景的策略。在本研究中，以中国春为背景的一个新的小麦-绒毛草T2DS·2V#4L易位系表现出对蜡状芽孢杆菌的稳定抗性。通过回交将T2DS·2V#4L染色体导入小麦品种爱康58，与NIL-T2DS·2DL相比，NIL-T2DS-2V#4L的尖眼病抗性显著增加，并且在BC2F2、BC2F2:3和BC2F2:4代中没有观察到2V#4 L对农艺性状的不利影响。2V#4L的流式排序测序产生384.3Mb的组装序列，并预测了8836个基因，其中6154个在CS的2AL、2BL和2DL臂中的至少一个臂中具有直向同源物，而1549个基因是2V#4L独有的。在10个已测序的面包小麦品种中，在2V#4L和2DL基因中检测到约100000个SNPs。开发了一种竞争性等位基因特异性聚合酶链反应和30个保守的直向同源序列标记来追踪小麦背景中的2V#4L染色质。T2DS·2V#4L补偿易位系代表了一种具有强烈眼斑抗性的新种质，该标记将允许在育种计划中快速检测。

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来源期刊

Crop Journal Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

9.90

自引率

3.00%

发文量

638

审稿时长

41 days

期刊介绍： The major aims of The Crop Journal are to report recent progresses in crop sciences including crop genetics, breeding, agronomy, crop physiology, germplasm resources, grain chemistry, grain storage and processing, crop management practices, crop biotechnology, and biomathematics. The regular columns of the journal are Original Research Articles, Reviews, and Research Notes. The strict peer-review procedure will guarantee the academic level and raise the reputation of the journal. The readership of the journal is for crop science researchers, students of agricultural colleges and universities, and persons with similar academic levels.