Expression of gastrin and cholecystokinin B receptor in Lateolabrax maculatus

Q1 Agricultural and Biological Sciences
Tingwen Cui , Jiaqi Wang , Zhongjun Hu , Xiaowu Chen
{"title":"Expression of gastrin and cholecystokinin B receptor in Lateolabrax maculatus","authors":"Tingwen Cui ,&nbsp;Jiaqi Wang ,&nbsp;Zhongjun Hu ,&nbsp;Xiaowu Chen","doi":"10.1016/j.aaf.2021.11.002","DOIUrl":null,"url":null,"abstract":"<div><p>Gastrin (gas) is a peptide hormone that stimulates gastric acid secretion by gastric parietal cells and stimulates gastric motility. The cholecystokinin B receptor (cckbr) can act as a receptor for gastrin, conveying regulatory information on gastrin, but there are fewer studies on its function in fish. The <em>Lateolabrax maculatus</em> is one of the marine aquaculture species in China, it widely distribute in coastal areas. In the study, we cloned the genes of <em>Lateolabrax maculatus</em> gastrin (Lm-gas) and <em>Lateolabrax maculatus</em> cholecystokinin B receptor (Lm-cckbr). The results showed that the full-length gene of Lm-gas is 638bp and the carboxy-terminal conserved domain (DFGRR) is the core functional domain of gastrin protein. The Lm-cckbr gene has a total nucleotide sequence of 2066 bp, and the open reading frame encodes a total of 453 amino acids. The result of protein sequence alignment showed that the similarity between Lm-cckbr protein and other different species was 50.11%–89.67%. The PCR results showed that Lm-gas and Lm-cckbr were expressed in brain and stomach. Further localization by immunehistochemical staining showed that Lm-gas protein was located in the mucosal layer of the gastric wall, but the expression signal was weak in the brain. Hunger causeed a significant decrease in these two genes. The results provided basic research data for further study on the function of Lm-gas and its recepter Lm-cckbr in the in the central nervous system and digestive system of <em>Lateolabrax maculatus</em>.</p></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Aquaculture and Fisheries","FirstCategoryId":"1091","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2468550X21001519","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 0

Abstract

Gastrin (gas) is a peptide hormone that stimulates gastric acid secretion by gastric parietal cells and stimulates gastric motility. The cholecystokinin B receptor (cckbr) can act as a receptor for gastrin, conveying regulatory information on gastrin, but there are fewer studies on its function in fish. The Lateolabrax maculatus is one of the marine aquaculture species in China, it widely distribute in coastal areas. In the study, we cloned the genes of Lateolabrax maculatus gastrin (Lm-gas) and Lateolabrax maculatus cholecystokinin B receptor (Lm-cckbr). The results showed that the full-length gene of Lm-gas is 638bp and the carboxy-terminal conserved domain (DFGRR) is the core functional domain of gastrin protein. The Lm-cckbr gene has a total nucleotide sequence of 2066 bp, and the open reading frame encodes a total of 453 amino acids. The result of protein sequence alignment showed that the similarity between Lm-cckbr protein and other different species was 50.11%–89.67%. The PCR results showed that Lm-gas and Lm-cckbr were expressed in brain and stomach. Further localization by immunehistochemical staining showed that Lm-gas protein was located in the mucosal layer of the gastric wall, but the expression signal was weak in the brain. Hunger causeed a significant decrease in these two genes. The results provided basic research data for further study on the function of Lm-gas and its recepter Lm-cckbr in the in the central nervous system and digestive system of Lateolabrax maculatus.

胃泌素和胆囊收缩素B受体在黄斑蝶组织中的表达
胃泌素(gas)是一种肽类激素,可刺激胃壁细胞分泌胃酸并刺激胃运动。胆囊收缩素B受体(cckbr)可以作为胃泌素的受体,传递胃泌素的调节信息,但对其在鱼类中的作用的研究较少。斑腹蛛是我国海洋养殖的一种,广泛分布于沿海地区。在本研究中,我们克隆了斑尾蛇胃泌素(Lm-gas)和斑尾蛇胆囊收缩素B受体(Lm-cckbr)的基因。结果表明,Lm-gas全长638bp,羧基末端保守结构域(DFGRR)是胃泌素蛋白的核心功能结构域。Lm-cckbr基因的总核苷酸序列为2066bp,开放阅读框共编码453个氨基酸。蛋白质序列比对结果表明,Lm-cckbr蛋白与其他不同物种的相似性为50.11%–89.67%。PCR结果显示,Lm-gas和Lm-cckbr在脑和胃中均有表达。免疫组织化学染色进一步定位显示,Lm气体蛋白位于胃壁粘膜层,但在大脑中表达信号较弱。饥饿导致这两个基因显著减少。研究结果为进一步研究Lm-gas及其受体Lm-cckbr在斑腹蛛中枢神经系统和消化系统中的作用提供了基础研究数据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Aquaculture and Fisheries
Aquaculture and Fisheries Agricultural and Biological Sciences-Aquatic Science
CiteScore
7.50
自引率
0.00%
发文量
54
审稿时长
48 days
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信