Detection and molecular characterization of Copamyntis obliquifasciella (Hampson, 1896) infesting medicinal plant, Cassia fistula L from India

IF 3.8 2区 农林科学 Q1 PLANT SCIENCES
K.T. Shivakumara , M.C. Keerthi , P.R. Shashank , J. Komal , A.C. Polaiah , R.S. Ramya , T. Venkatesan , D. Sagar , Ryan Casini , Ihab M. Moussa , Hosam O. Elansary , Ahmed M. El-Sabrout
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Abstract

Cassia fistula L. is a valuable medicinal plant utilized in many traditional medical systems, including Ayurveda and Oriental medicine system. Copamyntis obliquifasciella Hampson (Lepidoptera: Pyralidae) is a polyphagous insect pest, however the available literature of this pest on C. fistula is limited or rather scarce in India as well as world. Considering the economic importance of this medicinal plant, documentation of insects pests along with the bio-ecology and natural enemy fauna is important for formulating the integrated pest management program. The present study describes the incidence, damage and life cycle of C. obliquifasciella on C. fistula in India. The identity of the pest was confirmed through the examination of morphological characteristics of male and female genitalia, further confirmed through molecular analysis (GenBank no. MK559413). The phylogenetic analysis revealed that the Indian population of C. obliquifasciella shared a close relationship with the populations from Pakistan and China. The initial instar larvae of C. obliquifasciella is feed by nibbling and scraping the chlorophyll content of the leaves, and later they stitch two or three leaves together and fed in between those stitched leaves. The percent pest incidence varied between 20 and 100, and the foliar damage of 5.5–71% was recorded. The total life cycle of C. obliquifasciella was completed in 31.20 ± 2.14 (male) and 34.25 ± 2.36 (female) days. Two hymenopteran parasitoids, such as Phanerotoma sp. (Braconidae: Cheloninae) and Apanteles taragamae Viereck (Braconidae: Microgastrinae) were associated with C. obliquifasciella. To best of our knowledge, this is the first description of C. obliquifasciella and their associated natural enemies from the Indian region.

印度药用植物决明子(Cassia瘘管病)中斜交Copamyntis obliifasciella(Hampson,1896)的检测及其分子特征
决明是一种珍贵的药用植物,在许多传统医学系统中都有使用,包括阿育吠陀和东方医学系统。Copamyntis obliifasciella Hampson(鳞翅目:梨科)是一种多食性害虫,但在印度和世界范围内,关于这种害虫的瘘管病文献有限或相当稀少。考虑到这种药用植物的经济重要性,记录害虫以及生物生态学和天敌动物群对于制定综合害虫管理计划非常重要。本研究描述了印度产瘘管上斜孢梭菌的发病率、危害和生命周期。通过检查雄性和雌性生殖器的形态特征,进一步通过分子分析(GenBank no.MK559413)确认了该害虫的身份。系统发育分析表明,印度弯孢菌种群与巴基斯坦和中国的种群关系密切。斜孢C.obliifasciella的初龄幼虫通过蚕食和刮擦叶片的叶绿素含量来进食,然后它们将两三片叶子缝合在一起,并在缝合的叶子之间进食。虫害发生率在20至100之间变化,记录的叶面损伤为5.5至71%。斜交藻的总生命周期分别为31.20±2.14天(雄性)和34.25±2.36天(雌性)。两种膜翅目寄生蜂,如Phanerotoma sp.(Braconidae:Cheloninae)和Apanteles taragamae Viereck(Bracondae:Microgastrinae)与C.obliifasciella有关。据我们所知,这是对印度地区的C.obliifasciella及其相关天敌的首次描述。
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来源期刊
Journal of Applied Research on Medicinal and Aromatic Plants
Journal of Applied Research on Medicinal and Aromatic Plants Pharmacology, Toxicology and Pharmaceutics-Drug Discovery
CiteScore
6.40
自引率
7.70%
发文量
80
审稿时长
41 days
期刊介绍: JARMAP is a peer reviewed and multidisciplinary communication platform, covering all aspects of the raw material supply chain of medicinal and aromatic plants. JARMAP aims to improve production of tailor made commodities by addressing the various requirements of manufacturers of herbal medicines, herbal teas, seasoning herbs, food and feed supplements and cosmetics. JARMAP covers research on genetic resources, breeding, wild-collection, domestication, propagation, cultivation, phytopathology and plant protection, mechanization, conservation, processing, quality assurance, analytics and economics. JARMAP publishes reviews, original research articles and short communications related to research.
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