ISSR-assisted spatial genetic structure, population admixture, and biodiversity estimates across locally adopted saffron ecotypes from 18 different provenances of Iran

IF 3.8 2区 农林科学 Q1 PLANT SCIENCES
Seid M. Alavi-Siney , Jalal Saba , Alireza F. Siahpirani , Jaber Nasiri
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Abstract

Here, genetic variability and spatial genetic structure patterns governing on a 90-sample collection of Iranian saffron ecotype gathered from 18 provenances were scrutinized using 20 ISSR markers. 17 out of 20 ISSR primers exhibited polymorphism with 130 polymorphic amplicons from 4 to 15 bands per primer, with an average of 7.647. Considering maximum values of PIC (0.37), discrimination power (D; 0.71), and expected heterozygosity (H; 0.497), both ISSR-7 and ISSR-9 primers were nominated to generate maximal discrimination and heterozygosity. Based on phylogenetic tree and PCoA, the ecotypes were classified into two major groups, but inconsistent with the geographical distribution. Similarly, via applying Bayesian population structure clustering, two sub-populations (K = 2) were observed, four ecotypes were admixture (Q < 0.70), and the rest were pure ecotypes (Q ≥ 0.70). Sub-population 1 was less genetically diverse (FST = 0.1658) than sub-population 2 (FST = 0.5593). As to AMOVA, 56.563% and 43.436% of total genetic variation were partitioned into inter- and intra-population variation, respectively. Based on spatial PCA (sPCA), robust signals of “global” and “local” structures were recognized, only the former was statistically significant (p < 0.001). Significant IBD patterns were also observed via Mantel test calculated between original/unbiased Nei’s genetic distances and geographic distances (p < 0.001) across full dataset. The results revealed ISSR capabilities in saffron DNA fingerprinting and biodiversity assessment, moderate-high genetic variability alongside significant global spatial genetic structure among saffron ecotypes, followed by low levels of gene flow (Nm = 0.2801) and admixture patterns.

issr辅助的空间遗传结构、种群混合和生物多样性估计在伊朗18个不同种源的当地采用的藏红花生态型中
本文利用20个ISSR标记对从18个种源采集的90个伊朗藏红花生态型样品的遗传变异性和空间遗传结构模式进行了仔细研究。20个ISSR引物中有17个表现出多态性,每个引物有130个多态性扩增子,每个扩增子有4-15条带,平均7.647条。考虑到PIC的最大值(0.37)、辨别力(D;0.71)和预期杂合度(H;0.497),ISSR-7和ISSR-9引物被提名产生最大辨别力和杂合度。根据系统发育树和PCoA,将生态型分为两大类,但与地理分布不一致。同样,通过应用贝叶斯群体结构聚类,观察到两个亚群体(K=2),四个生态型为混合型(Q<;0.70),其余为纯生态型(Q≥0.70)。亚群体1的遗传多样性(FST=0.1658)低于亚群体2(FST=0.5593),56.563%和43.436%的遗传变异分为群体间变异和群体内变异。基于空间主成分分析(sPCA),识别出了“全局”和“局部”结构的稳健信号,只有前者具有统计学意义(p<;0.001)。通过Mantel检验,在整个数据集的原始/无偏Nei遗传距离和地理距离(p<:0.001)之间计算,也观察到了显著的IBD模式。结果显示,ISSR在藏红花DNA指纹图谱和生物多样性评估中具有能力,在藏红花生态型中具有中等高的遗传变异性和显著的全球空间遗传结构,其次是低水平的基因流(Nm=0.2801)和混合模式。
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来源期刊
Journal of Applied Research on Medicinal and Aromatic Plants
Journal of Applied Research on Medicinal and Aromatic Plants Pharmacology, Toxicology and Pharmaceutics-Drug Discovery
CiteScore
6.40
自引率
7.70%
发文量
80
审稿时长
41 days
期刊介绍: JARMAP is a peer reviewed and multidisciplinary communication platform, covering all aspects of the raw material supply chain of medicinal and aromatic plants. JARMAP aims to improve production of tailor made commodities by addressing the various requirements of manufacturers of herbal medicines, herbal teas, seasoning herbs, food and feed supplements and cosmetics. JARMAP covers research on genetic resources, breeding, wild-collection, domestication, propagation, cultivation, phytopathology and plant protection, mechanization, conservation, processing, quality assurance, analytics and economics. JARMAP publishes reviews, original research articles and short communications related to research.
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