The role of anti-E. coli antibody from maternal colostrum on the colonization of newborn dairy calves gut with Escherichia coli and the development of clinical diarrhea

Abstract

Escherichia coli (E. coli) is an early organism in the colonization of mucosal tissue and has an influence on the development of mucosal and systemic inflammatory responses. To understand the impact of transfer of Immunoglobulin G (IgG), E. coli-specific antibody and general immune factors to the calf on the gut microbiota, an observational study that followed the temporal colonization of the intestine with E. coli as correlated with the total quantity of IgG and the antibody recognizing E. coli, was conducted in newborn Holstein calves. The calves were subjected to the “gold standard” of colostrum management, including dam vaccination prior to delivery to prime colostrum. Twenty Holstein dams, producing an adequate quantity of good-quality colostrum following uncomplicated deliveries of healthy female offspring, were utilized in this study. Each calf was monitored and scored daily for diarrhea. Blood and fecal samples were collected from calves on day (D) D1 (before colostrum intake), then D3, D7, D14 and D28 after colostrum feeding. E. coli isolated from fecal samples of the calves were assessed for virulence genes and the quantity per gram of feces using conventional Polymerase chain reaction and Real-time polymerase chain reaction quantitative, respectively. The total quantity of plasma IgG and the titer of E. coli-binding antibody were measured by enzyme-linked immunosorbent assay. The peak in diarrhea in the calves was observed on D12. No IgG or E. coli-binding antibody were detected prior to colostrum intake (D1). Both total IgG and binding antibody sharply increased by D3. Total and binding IgG declined with calf age. The total number of bacteria (16S rRNA) in feces was similar between D1 and D14. The number of total bacteria increased over the period from D14 to D28. The number of E. coli was minimal on D1. The number of E. coli increased during the period D3-D7. A decrease in the number of E. coli per gram of feces declines between D14and D28. The stable toxin gene was frequently identified in the E. coli isolated from the calves. Further, E. coli K99 was not identified in this study. While the gut was colonized by E. coli early in life corresponding to the peak level of IgG and specific antibody, a decreasing number of E. coli were observed as the passive immunity waned. This suggests that the function of the passive antibody may be to control colonization and seed innate immune function that then stabilizes the level of E. coli in the digestive tract of calves.