Phenylalanine-Arginine-β-Naphthylamide Enhances the Photobactericidal Effect of Methylene Blue on Pseudomonas aeruginosa.

IF 1.8 Q2 SURGERY

Photobiomodulation, photomedicine, and laser surgery Pub Date : 2023-10-01 DOI:10.1089/photob.2023.0005

Lixia He, Shanlin Yang, Weijun Xuan, Xiumei Zhen, Qi Qi, Yongqi Qi, Qingxiang Li, Meixia Du, Michael R Hamblin, Liyi Huang

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Abstract

Objective: To investigate the effectiveness, dosing sequence, concentration, and mechanism of antimicrobial photodynamic inactivation (aPDI) using methylene blue (MB) plus phenylalanine-arginine-β-naphthylamide (PAβN) against Pseudomonas aeruginosa. Methods: P. aeruginosa bacterial suspension was incubated with MB for different times (5-240 min), and then, 10 J/cm² red light was irradiated. The efflux pump inhibitor (EPI) PAβN (10-100 μg/mL) was combined with MB (1-20 μM) in different sequences (PAβN-first, PAβN+MB, PAβN-after). Colony-forming units were then determined by serial dilution. Results: Using MB 10 μM plus 10 J/cm², the killing effect of MB-aPDI on P. aeruginosa increased first and then decreased with longer incubation time. The killing effect of MB+PAβN-aPDI on P. aeruginosa was better than that of MB-aPDI (p < 0.05) by up to 2 logs. PAβN-first had the best killing effect, whereas PAβN-after had the worst killing effect. The killing effect increased with PAβN concentration and at 100 μg/mL reached 5.1 logs. Conclusions: The EPI PAβN enhanced the bactericidal effect of MB-aPDI on P. aeruginosa, especially when added before MB. It is proposed that MB is a substrate of the resistance-nodulation-division family efflux pump.

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苯丙氨酸精氨酸-β-萘酰胺增强亚甲基蓝对铜绿假单胞菌的光杀菌作用。

目的：研究亚甲蓝（MB）加苯丙氨酸精氨酸-β-萘酰胺（PAβN）对铜绿假单胞菌的光动力灭活效果、给药顺序、浓度和机制。方法：将铜绿假单胞菌悬浮液与MB孵育不同时间（5-240 分钟），然后，10 照射J/cm2的红光。外排泵抑制剂（EPI）PAβN（10-100 μg/mL）与MB（1-20 μM）在不同序列中（PAβN-第一，PAβN+MB，PAβN-之后）。然后通过连续稀释来确定菌落形成单位。结果：使用MB 10 μM加10 J/cm2时，MB-aPDI对铜绿假单胞菌的杀伤作用随培养时间的延长先增加后降低。βN-PDI对铜绿假单胞菌的杀灭效果优于MB-aPDI（P 结论：EPI-PAβN增强了MB-aPDI对铜绿假单胞菌的杀菌作用，尤其是在MB前添加。提出MB是电阻结瘤分裂家族外排泵的基质。

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来源期刊

Photobiomodulation, photomedicine, and laser surgery

CiteScore

4.10

自引率

0.00%

发文量

期刊介绍： Photobiomodulation, Photomedicine, and Laser Surgery Editor-in-Chief: Michael R Hamblin, PhD Co-Editor-in-Chief: Heidi Abrahamse, PhD