Down-regulation of the FTO gene in follicular fluid of infertile women with ovarian endometriosis.

IF 2 4区医学 Q3 ENDOCRINOLOGY & METABOLISM

Gynecological Endocrinology Pub Date : 2023-12-01 Epub Date: 2023-10-23 DOI:10.1080/09513590.2023.2269273

Yanmei Li, Naihui Wang, Yuanxue Jing, Jiajing He, Fangfang Li, Xuehong Zhang

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引用次数: 0

Abstract

Objective: To evaluate FTO concentrations in follicular fluid (FF) of women with ovarian endometriosis (OE) and controls women without OE undergoing in vitro fertilization-embryo transfer (IVF-ET).

Methods: FTO concentrations in FF were measured in 74 patients (37 in the control group and 37 in the OE group) by ELISA. We measured the expression of FTO in GCs of 40 patients (19 in the control group and 21 in the OE group) by RT-qPCR. The level of m⁶A in GCs was measured in 20 patients (10 in the control group and 10 in the OE group) by colorimetry.

Results: Compared with the control group, FTO concentrations in FF (6.92 ± 0.44 vs. 5.67 ± 0.40 ng/ml) (p <.05) and FTO mRNA level in GCs of OE group were decreased significantly (p <.05), and the level of m⁶A was increased (0.21 ± 0.01 vs. 0.17 ± 0.03 ng) (p >.05).

Conclusions: The FTO concentrations in FF of infertility women with OE are decreased, which may be related to the impaired oocyte quality in endometriosis patients.

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卵巢子宫内膜异位症不孕妇女卵泡液中FTO基因的下调。

目的：评价卵巢子宫内膜异位症（OE）患者和非OE患者接受体外受精-胚胎移植（IVF-ET）时卵泡液中FTO的浓度。方法：用ELISA法测定74例（对照组37例，OE组37例）卵泡液中的FTO浓度。我们通过RT-qPCR测量了40名患者（对照组19名，OE组21名）GC中FTO的表达。用比色法测定了20名患者（对照组10名，OE组10名）GC中m6A的水平。结果：与对照组相比，FF中FTO浓度（6.92 ± 0.44对5.67 ± 0.40 ng/ml）（p p6A增加（0.21 ± 0.01对0.17 ± 0.03 结论：患有OE的不孕妇女FF中FTO浓度降低，这可能与子宫内膜异位症患者卵母细胞质量受损有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Gynecological Endocrinology 医学-妇产科学

CiteScore

4.40

自引率

5.00%

发文量

137

审稿时长

3-6 weeks

期刊介绍： Gynecological Endocrinology , the official journal of the International Society of Gynecological Endocrinology, covers all the experimental, clinical and therapeutic aspects of this ever more important discipline. It includes, amongst others, papers relating to the control and function of the different endocrine glands in females, the effects of reproductive events on the endocrine system, and the consequences of endocrine disorders on reproduction