A molecular and morphological study of Ascaris suum in a human-pig contact scenario in northeastern Brazil.

IF 1.3 4区农林科学 Q2 Veterinary

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2023-10-13 eCollection Date: 2023-01-01 DOI:10.1590/S1984-29612023057

Polyanna Araújo Alves Bacelar, Jéssica Pereira Dos Santos, Deiviane Aparecida Calegar, Denilson de Araújo E Silva, Daniella Nobre Leal, Brenda Bulsara Costa Evangelista, Elis Regina Chaves Dos Reis, Jacenir Reis Dos Santos Mallet, Filipe Anibal Carvalho-Costa, Lauren Hubert Jaeger, Kerla Joeline Lima Monteiro

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Abstract

The aim of the present study was to assess morphologic and genetic data on ascariasis in swine (Sus scrofa domesticus) and humans in low-resource rural and periurban communities in the state of Piauí, Brazil. Our cross-sectional survey included 100 fecal samples obtained from swine and 682 samples from humans. Fifteen pigs were necropsied. Human and porcine fecal samples were examined to identify Ascaris eggs. Parasites obtained in the swine necropsies were studied using scanning electron microscopy (SEM), and the mitochondrial gene encoding the cytochrome oxidase 1 (cox1) enzyme was partially amplified and sequenced for molecular taxonomy and phylogenetic analyses. The overall prevalence of Ascaris eggs in the swine fecal samples was 16/100 (16%). No Ascaris eggs were identified in the human fecal samples. SEM of six worms recovered from pigs demonstrated morphological characteristics of A. suum. Cox1 sequences were compatible with A. suum reference sequences. Original and reference (GenBank) nucleotide sequences were organized into clusters that did not segregate the parasites by host species or and region. The largest haplogroups were dominated by haplotypes H01, H02 and H31. In the communities studied, there was no epidemiological evidence of the zoonotic transmission of ascariasis at the human-swine interface.

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巴西东北部人猪接触场景中猪蛔虫的分子和形态学研究。

本研究的目的是评估巴西皮亚伊州低资源农村和城市周边社区猪（Sus scrofa domesticus）和人类蛔虫的形态学和遗传学数据。我们的横断面调查包括100份从猪身上获得的粪便样本和682份从人类身上获取的样本。对15头猪进行了尸检。对人和猪的粪便样本进行检查，以鉴定蛔虫卵。利用扫描电子显微镜（SEM）对猪尸检中获得的寄生虫进行了研究，并对编码细胞色素氧化酶1（cox1）酶的线粒体基因进行了部分扩增和测序，以进行分子分类学和系统发育分析。猪粪便样本中蛔虫卵的总患病率为16/100（16%）。在人类粪便样本中未发现蛔虫卵。从猪身上回收的六种蠕虫的SEM显示了A.suum的形态特征。Cox1序列与A.suum参考序列相容。原始和参考（GenBank）核苷酸序列被组织成簇，不按宿主物种或区域分离寄生虫。最大的单倍型群以H01、H02和H31单倍型为主。在所研究的社区中，没有流行病学证据表明蛔虫病在人-猪界面传播。

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来源期刊

Revista Brasileira De Parasitologia Veterinaria PARASITOLOGY-VETERINARY SCIENCES

CiteScore

2.60

自引率

7.70%

发文量

审稿时长

>12 weeks

期刊介绍： La revista es un órgano de difusión del Colegio Brasileño de Parasitología Veterinaria, con una especificidad dentro de esa área, la difusión de los resultados de la investigación brasileña en las áreas de Helmintología, Protozoología, Entomología y agentes transmitidos por artrópodos, relacionados con la salud animal.