Transcriptome analysis of Apis mellifera antennae reveals molecular divergence underlying the division of labour in worker bees

IF 2.3 2区 农林科学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Bairu Liu, Yicong Xu, Weixing Zhang
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Abstract

The olfactory system plays a fundamental role in mediating insect behaviour. Worker bees exhibit an age-dependent division of labour, performing discrete sets of behaviours throughout their lifespan. The behavioural states of bees rely on their sense of the environment and chemical communication via their olfactory system, the antennae. However, the olfactory adaptation mechanism of worker bees during their behavioural development remains unclear. In this study, we conducted a comprehensive and quantitative analysis of antennal gene expression in the Apis mellifera of newly emerged workers, nurses, foragers and defenders using RNA-seq. We found that the antenna tissues of honey bees continued developing after transformation from newly emerged workers to adults. Additionally, we identified differentially expressed genes associated with bee development and division of labour. We validated that major royal jelly protein genes are highly and specifically expressed in nurse honey bee workers. Furthermore, we identified and validated significant alternative splicing events correlated with the development and division of labour. These findings provide a comprehensive transcriptome profile and a new perspective on the molecular mechanisms that may underlie the worker honey bee division of labour.

Abstract Image

蜜蜂触角的转录组分析揭示了工蜂分工的分子差异。
嗅觉系统在调节昆虫行为中起着重要作用。工蜂表现出与年龄相关的分工,在其一生中表现出一系列离散的行为。蜜蜂的行为状态依赖于它们对环境的感知和通过嗅觉系统(触角)进行的化学交流。然而,工蜂在行为发育过程中的嗅觉适应机制尚不清楚。在这项研究中,我们使用RNA-seq对新出现的工人、护士、觅食者和保卫者的蜜蜂触角基因表达进行了全面和定量的分析。我们发现,蜜蜂的触角组织在从新出现的工蜂转变为成虫后继续发育。此外,我们还鉴定了与蜜蜂发育和分工相关的差异表达基因。我们验证了主要蜂王浆蛋白基因在蜜蜂护理工作者中高度特异性表达。此外,我们确定并验证了与发育和分工相关的重要替代剪接事件。这些发现提供了一个全面的转录组图谱,并为工蜂分工的分子机制提供了新的视角。
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来源期刊
Insect Molecular Biology
Insect Molecular Biology 生物-昆虫学
CiteScore
4.80
自引率
3.80%
发文量
68
审稿时长
6-12 weeks
期刊介绍: Insect Molecular Biology has been dedicated to providing researchers with the opportunity to publish high quality original research on topics broadly related to insect molecular biology since 1992. IMB is particularly interested in publishing research in insect genomics/genes and proteomics/proteins. This includes research related to: • insect gene structure • control of gene expression • localisation and function/activity of proteins • interactions of proteins and ligands/substrates • effect of mutations on gene/protein function • evolution of insect genes/genomes, especially where principles relevant to insects in general are established • molecular population genetics where data are used to identify genes (or regions of genomes) involved in specific adaptations • gene mapping using molecular tools • molecular interactions of insects with microorganisms including Wolbachia, symbionts and viruses or other pathogens transmitted by insects Papers can include large data sets e.g.from micro-array or proteomic experiments or analyses of genome sequences done in silico (subject to the data being placed in the context of hypothesis testing).
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