Unraveling the molecular mechanisms of selenite reduction: transcriptomic analysis of Bacillus reveals the key role of sulfur assimilation.

IF 2 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology Letters Pub Date : 2023-12-01 Epub Date: 2023-10-21 DOI:10.1007/s10529-023-03439-y

Ying Yang, Jiawei Jing, Shuling Fan, Zhuo Chen, Yuanyuan Qu

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Abstract

Selenite biotransformation by microorganisms is an effective detoxification and assimilation process. However, current knowledge of the molecular mechanisms of selenite reduction remains circumscribed. Here, the reduction of Se(IV) by a highly selenite-resistant Bacillus sp. SL (up to 50 mM) was systematically analyzed, and the molecular mechanisms of selenite reduction were investigated. Remarkably, 10 mM selenite was entirely transformed by the strain SL within 20 h, demonstrating a faster conversion rate compared to other microorganisms. Furthermore, glutathione (GSH) and exopolysaccharides (EPS) changes were also monitored during the process. Transcriptomic analysis revealed that the genes of ferredoxin-sulfite oxidoreductase (6.82) and sulfate adenylyltransferase (6.32) were significantly upregulated, indicating that the sulfur assimilation pathway is the primary reducing pathway involved in selenite reduction by strain SL. Moreover, key genes associated with NAD(P)/FAD-dependent oxidoreductases and thioredoxin were significantly upregulated. The reduction of Se(IV) was mediated by multiple pathways in strain SL. To our knowledge, this is the initial report to identify the involvement of sulfur assimilation pathway in selenite reduction for bacillus, which is rare in aerobic bacteria.

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揭示亚硒酸盐还原的分子机制：芽孢杆菌的转录组学分析揭示了硫同化的关键作用。

微生物对亚硒酸盐的生物转化是一个有效的解毒和同化过程。然而，目前对亚硒酸盐还原的分子机制的认识仍然受到限制。在此，系统分析了高亚硒酸盐抗性芽孢杆菌SL（高达50mM）对Se（IV）的还原，并研究了亚硒酸还原的分子机制。值得注意的是，菌株SL在20小时内完全转化了10mM亚硒酸盐，与其他微生物相比，转化率更快。此外，还监测了谷胱甘肽（GSH）和胞外多糖（EPS）的变化。转录组学分析显示，铁氧还蛋白亚硫酸氧化还原酶（6.82）和硫酸腺苷酸转移酶（6.32）基因显著上调，表明硫同化途径是SL菌株还原亚硒酸盐的主要还原途径，与NAD（P）/FAD依赖性氧化还原酶和硫氧还蛋白相关的关键基因显著上调。菌株SL中Se（IV）的还原是由多种途径介导的。据我们所知，这是确定硫同化途径参与芽孢杆菌亚硒酸盐还原的初步报告，而芽孢杆菌在需氧菌中很少见。

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来源期刊

Biotechnology Letters 工程技术-生物工程与应用微生物

CiteScore

5.90

自引率

3.70%

发文量

108

审稿时长

1.2 months

期刊介绍： Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them. All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included. Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields. The emphasis is very much on novelty and immediacy in order to justify rapid publication of authors’ results. It should be noted, however, that we do not normally publish papers (but this is not absolute) that deal with unidentified consortia of microorganisms (e.g. as in activated sludge) as these results may not be easily reproducible in other laboratories. Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.