Indole-3-acetic acid impacts biofilm formation and virulence production of Pseudomonas aeruginosa.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2023-08-01 Epub Date: 2023-11-20 DOI:10.1080/08927014.2023.2269537

Sudharshini Jayaraman, Monika Adhilaxmi Kannan, Nandhini Rajendhran, Georrge J John, Thirumurugan Ramasamy

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引用次数: 0

Abstract

Bacterial pathogenesis involves complex mechanisms contributing to virulence and persistence of infections. Understanding the multifactorial nature of bacterial infections is crucial for developing effective interventions. The present study investigated the efficacy of indole-3-acetic acid (IAA) against Pseudomonas aeruginosa with various end points including antibacterial activity, minimum inhibitory concentration (MIC), virulence factor production, biofilm inhibition, bacterial cell detachment, and viability assays. Results showed significant biofilm inhibition, bacterial cell detachment, and modest effects on bacterial viability. Microscopic analysis confirmed the disintegrated biofilm matrix, supporting the inhibitory effect of IAA. Additionally, molecular docking studies revealed potential mechanisms of action through active bond interactions between IAA and virulence proteins. These findings highlight IAA as an effective antibiofilm agent against P. aeruginosa.

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吲哚-3-乙酸影响铜绿假单胞菌的生物膜形成和毒力产生。

细菌的发病机制涉及导致毒力和感染持续性的复杂机制。了解细菌感染的多因素性质对于制定有效的干预措施至关重要。本研究考察了吲哚-3-乙酸（IAA）对铜绿假单胞菌的疗效，包括抗菌活性、最低抑菌浓度（MIC）、毒力因子产生、生物膜抑制、细菌细胞分离和活力测定。结果显示出显著的生物膜抑制、细菌细胞分离以及对细菌活力的适度影响。显微镜分析证实了分解的生物膜基质，支持IAA的抑制作用。此外，分子对接研究通过IAA和毒力蛋白之间的活性键相互作用揭示了潜在的作用机制。这些发现突出表明IAA是一种有效的抗铜绿假单胞菌的抗菌膜剂。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464