Chitosan modified graphene field-effect transistor biosensor for ultrasensitive procalcitonin detection

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta Pub Date : 2023-10-13 DOI:10.1016/j.talanta.2023.125308

Furong Chen , Ying Zhang , Mingxuan Wang , Jinghai Liu , Wenfeng Hai , Yushuang Liu

引用次数: 0

Abstract

Sepsis is a systemic inflammatory response caused by a bacterial infection that often leading to tissue damage, organ failure and death. Procalcitonin (PCT), as a peptide precursor to hormones, is the main biomarker to identification of the sepsis. In this study, a chitosan modified graphene field transistor (CTS-GFET) was established and first time used for PCT ultra-sensitive detection. CTS was functionalized on the GFET channel surface to immobilized anti-PCT by glutaraldehyde. This biosensor exhibited the detection limit as low as 0.82 ag/mL in PBS, which exhibited 3 times enhancement compared with GFET biosensors. The enhancement mechanisms of CTS-GFET were studied by electrical theoretical model. In addition, the CTS-GFET biosensor was successfully applied to quantify the concentration of the PCT in human serum samples, indicating the potential use in clinical application.

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壳聚糖修饰石墨烯场效应晶体管生物传感器用于超灵敏的降钙素原检测。

败血症是由细菌感染引起的全身炎症反应，通常会导致组织损伤、器官衰竭和死亡。降钙素原（PCT）作为激素的前体肽，是鉴定败血症的主要生物标志物。本研究建立了壳聚糖修饰石墨烯场晶体管（CTS-GFET），并首次用于PCT超灵敏检测。用戊二醛将CTS功能化于GFET通道表面，固定化抗PCT。该生物传感器在PBS中的检测极限低至0.82 ag/mL，与GFET生物传感器相比提高了3倍。采用电学理论模型研究了CTS-GFET的增强机理。此外，CTS-GFET生物传感器已成功应用于定量人类血清样品中PCT的浓度，表明其在临床应用中的潜在用途。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.