A study involving PC-3 cancer cells and novel carbamate gemini surfactants: Is zeta potential the key to control adhesion to cells?

Q1 Engineering
R.V. Pavlov, G.A. Gaynanova, D.M. Kuznetsov, Ya.A. Ivanov, S.K. Amerkhanova, A.P. Lyubina, A.D. Voloshina, L.Ya. Zakharova
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引用次数: 4

Abstract

Liposome surface potential effect on cellular uptake and cytotoxicity is evaluated using liposomes, modified with cationic lipid DOTAP, a series of cationic gemini surfactants with two carbamate fragments, and an amphiphilic peptide SSRGD. The surfactants used are novel representatives of the gemini family with improved self-assembling activity coupled with potential biodegradable properties and displayed increasing antibacterial activity and cytotoxicity with the shortening of hydrophobic alkyl tails. The longest alkyl tail surfactant, 14-6-14(Et), was the most biocompatible of the series, which was chosen for liposome modification. Prepared liposomes of various compositions are characterized from morphological and physicochemical standpoints in order to optimize their biocompatibility and stability. The carbamate gemini surfactants were also twice as effective at providing positive charge to liposomes and less toxic compared to DOTAP. On their own, carbamate surfactants were able to increase cellular uptake of liposomes by 190%. The mixed composition of 14-6-14(Et) surfactant and SSRGD amphiphilic peptide was the most readily absorbed formulation among different tested neutral, cationic and RGD-modified liposomes. The comparison between the cellular uptake promotion is conducted as to what is the most selective and efficient approach to enhance lipid nanoparticle uptake by cancerous cells.

一项涉及PC-3癌症细胞和新型氨基甲酸酯双子表面活性剂的研究:ζ电位是控制细胞粘附的关键吗?
脂质体表面电位对细胞摄取和细胞毒性的影响通过脂质体、阳离子脂质DOTAP、一系列带有两个氨基甲酸酯片段的阳离子gemini表面活性剂和两亲性肽SSRGD进行了评估。所使用的表面活性剂是gemini家族的新代表,具有改进的自组装活性以及潜在的生物降解性能,并且随着疏水烷基尾部的缩短而显示出增强的抗菌活性和细胞毒性。最长的烷基尾表面活性剂14-6-14(Et)是该系列中生物相容性最好的,被选择用于脂质体修饰。为了优化其生物相容性和稳定性,从形态和理化角度对制备的各种成分的脂质体进行了表征。氨基甲酸酯双子星表面活性剂为脂质体提供正电荷的效率是DOTAP的两倍,毒性更小。就其本身而言,氨基甲酸酯表面活性剂能够将脂质体的细胞摄取增加190%。其中,14-6-14(Et)表面活性剂与SSRGD两亲肽的混合配方是中性、阳离子和rgd修饰脂质体中最易吸收的配方。比较了促进细胞摄取的方法,以确定哪种方法最具选择性和最有效地增强了癌细胞对脂质纳米颗粒的摄取。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Smart Materials in Medicine
Smart Materials in Medicine Engineering-Biomedical Engineering
CiteScore
14.00
自引率
0.00%
发文量
41
审稿时长
48 days
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