Effect of Multi-Functional Therapeutic Active Extract of Marine Asterias rubens against Tooth Decaying Pathogens

Abstract

Recently, childhood caries have become one of the main health related diseases , especially developed by lactic acid bacteria, affecting millions of school going children worldwide. In the present work, we have isolated about 50 lactic acid bacteria samples from different tooth decay affected children from Fen Dental Clinic, Tirupur India. The emerging bacteria were confirmed by phenotype and genotype characterization. After, the pathogens were screened for the production of biofilm it was checked for the resistant mechanism of the Lactobacillus acidophilus. In this study the MTLA1-50 isolates produced strong biofilms which were confirmed for the Lactobacillus acidophilus carrying resistant capability. In particularly, the maximum strong biofilm producing strains were MTLA 32 and MTLA 50, followed by other isolates. As star fish extract has been reported to play a vital role in eliminating the bacterial biofilm for this motive in this study we have chosen the star fish, Asterias rubens extract in the concentration of 50, 100, 150 µl. The analysis was performed and executed using well diffusion standard techniques. The results showed that the strain No. MTLA 32 and MTLA 50 got the maximum zone of inhibition at 150 µl (52mm and 38 mm) of the star fish extract. The findings demonstrate that the star fish extract has a powerful anti-biotic ability to treat the dental pathogens particularly, caused by Lactobacillus acidophilus.