Anogeissus leiocarpus (DC.) Guill and Perr ameliorates pentylenetetrazole-induced seizure/cognitive impairment in rats via inhibition of oxidative stress

IF 1.8 Q3 PHARMACOLOGY & PHARMACY
Hauwa Adamu Audu, Amina Ahmed, Joseph Vandi Zirahei, Nathan Isaac Dibal, Samaila Musa Chiroma
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引用次数: 1

Abstract

The study investigates the role of Anogeissus leiocarpus methanol stem bark extract (ALSE) on seizure, oxidative stress and cognitive performance in pentylenetetrazole (PTZ)-induced epilepsy in rat model. Thirty Wistar rats were allocated into five groups (n = 6). Groups 1 and 2 received normal saline intra-peritoneal (i.p) every day and PTZ (i.p) at 35 mg/kg every other day respectively. Groups 3–5 were given ALSE orally at (250 mg/kg and 500 mg/kg) and Diazepam at 4 mg/kg (i.p) respectively. Groups 3–5 were given PTZ (i.p) at 35 mg/kg every other day for 30 days, 30 min after ALSE and Diazepam administration. The rats were observed for seizure activities and also evaluated for cognitive functions. The rats were euthanized thereafter and the brain histology and oxidative stress biomarkers were evaluated. PTZ induction resulted into increased seizure activities leading to the development of kindling, oxidative stress, cognitive impairment and histological aberration of the hippocampus. However, pretreatment with ALSE decreased seizure activities, reversed oxidative stress and cognitive impairment and preserved hippocampal histology relative to the PTZ alone treated rats. Conclusively, ALSE was found to increase seizure latency, prevented cognitive decline, and decreased seizure activities induced by PTZ-kindling in rats. Additionally, ALSE ameliorates PTZ-induced oxidative stress and histological aberrations of the hippocampus. Hence, this study proposed that ALSE might be a promising tool for ameliorating seizure in epilepsy.

Abstract Image

莱卡氏厌食症(DC)Guill和Perr通过抑制氧化应激改善大鼠戊四唑诱导的癫痫发作/认知障碍
本研究探讨了黑杖甲醇茎皮提取物(ALSE)对戊四唑(PTZ)致痫大鼠癫痫发作、氧化应激和认知能力的影响。Wistar大鼠30只,随机分为5组(n = 6)。1组和2组分别每天腹腔注射生理盐水(i.p)和PTZ (i.p),剂量为35 mg/kg,每隔一天注射一次。3 ~ 5组患者分别口服ALSE (250 mg/kg、500 mg/kg)和地西泮(4 mg/kg)。3 ~ 5组患者给予PTZ (i.p),剂量为35 mg/kg,每隔一天给药30 d,在ALSE和地西泮给药后30 min。观察大鼠的癫痫发作活动,并评估其认知功能。随后对大鼠实施安乐死,并对其进行脑组织组织学和氧化应激生物标志物的评价。PTZ诱导导致癫痫发作活动增加,导致点燃、氧化应激、认知障碍和海马组织畸变的发展。然而,与单独使用PTZ治疗的大鼠相比,ALSE预处理降低了癫痫发作活动,逆转了氧化应激和认知障碍,并保留了海马组织。最后,我们发现ALSE增加了大鼠的癫痫发作潜伏期,防止了认知能力下降,并减少了ptz点燃引起的癫痫发作活动。此外,ALSE还能改善ptz诱导的氧化应激和海马组织畸变。因此,本研究提出ALSE可能是一种有希望的改善癫痫发作的工具。
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来源期刊
Advances in Traditional Medicine
Advances in Traditional Medicine PHARMACOLOGY & PHARMACY-
CiteScore
4.30
自引率
0.00%
发文量
50
期刊介绍: Advances in Traditional Medicine (ADTM) is an international and peer-reviewed journal and publishes a variety of articles including original researches, reviews, short communications, and case-reports. ADTM aims to bridging the gap between Traditional knowledge and medical advances. The journal focuses on publishing valid, relevant, and rigorous experimental research and clinical applications of Traditidnal Medicine as well as medical classics. At the same time, the journal is devoted to communication among basic researcher and medical clinician interested in the advancement of Traditional Medicine. Topics covered by the journal are: Medical Classics & History; Biomedical Research; Pharmacology & Toxicology of Natural Products; Acupuncture & Moxibustion; Sasang Constitutional Medicine; Diagnostics and Instrumental Development; Clinical Research. ADTM is published four times yearly. The publication date of this journal is 30th March, June, September, and December.
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