Antimicrobial activity of secondary metabolites produced by Aspergillus neobridgeri isolated from Pistacia lentiscus against multi-drug resistant bacteria

IF 0.9 4区医学 Q4 PHARMACOLOGY & PHARMACY

Bangladesh Journal of Pharmacology Pub Date : 2020-07-23 DOI:10.3329/bjp.v15i3.40923

Nouari Sadrati, A. Zerroug, Rasime Demirel, Sabrina Bakli, D. Harzallah

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引用次数: 3

Abstract

The in vitro antibacterial activity of the endophytic fungus Aspergillus neobridgeri PLR9 (isolated from the plant Pistacia lentiscus) against a wide range of tested bacteria were studied. The maximum inhibitory activity was found on malt extract agar medium with a mean inhibitory zone of 11.3 mm (p<0.05). The ethyl acetate extract showed a significant inhibitory zone of 13.4 mm (p<0.05), followed by dichloromethane 9.1 mm. Whereas, n-hexane extract showed no activity. The largest inhibition zones were observed against Staphylococcus aureus 3, S. aureus 5 and Escherichia coli 1 with 21.0 mm of diameter. The MIC's were ranged between 312.5 and 1,250 μg/mL. The MBC's were ≤2500 μg/mL for most Gram positive bacteria and ≥2500 μg/mL for most Gram negative bacteria. Time kill assay study against the Enterococcus faecium showed a bactericidal effect at 4x MIC and bacteriostatic against Acinetobacter baumannii at different concentrations. Moreover, it is the first report, PLR9 isolated as endophyte and studied for their antimicrobial activity.

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新桥曲霉菌次级代谢产物对多药耐药菌的抗菌活性

研究了内生真菌新桥曲霉PLR9（从植物黄连木中分离）对多种受试细菌的体外抗菌活性。在麦芽提取物琼脂培养基上发现最大的抑制活性，平均抑制区为11.3mm（p<0.05）。乙酸乙酯提取物显示出13.4 mm的显著抑制区（p<0.05），其次是二氯甲烷9.1 mm。而正己烷提取物没有显示出活性。对直径为21.0mm的金黄色葡萄球菌3、金黄色葡萄杆菌5和大肠杆菌1观察到最大的抑制区。MIC在312.5和1250μg/mL之间。大多数革兰氏阳性菌的MBC≤2500μg/mL，大多数革兰氏阴性菌的MBCs≥2500μg/mL。对粪肠球菌的时间杀伤试验表明，在4倍MIC下具有杀菌作用，在不同浓度下对鲍曼不动杆菌具有抑菌作用。此外，这也是首次报道PLR9作为内生菌分离并研究其抗菌活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bangladesh Journal of Pharmacology PHARMACOLOGY & PHARMACY-

CiteScore

1.50

自引率

18.80%

发文量

审稿时长

>12 weeks

期刊介绍： Bangladesh Journal of Pharmacology (Bangladesh J Pharmacol) is an open access, video component, peer-reviewed journal of the Bangladesh Pharmacological Society (BDPS). A scholarly publication is defined ''open access'' when there are no financial, legal or technical barriers to accessing it. Anyone can read, download, copy, distribute, print or use it in educational purpose within the legal agreements. Peer review of a manuscript means the evaluation of research work by one or more people with similar competences. It can be open or blind. We do the single-blind peer review. Our readers want to know the methodology used by the researchers which is very important to get the results. The video clip of one of the methodologies is prepared by the authors and submit it to publish.