Electroanalytical determination of reduced-glutathione in biological samples

Journal of Clinical and Analytical Medicine Pub Date : 2019-05-01 DOI:10.4328/JCAM.6155

E. Savan

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引用次数: 0

Abstract

DOI: 10.4328/JCAM.6155 Received: 07.01.2019 Accepted: 14.01.2019 Published Online: 14.01.2019 Printed: 01.05.2019 J Clin Anal Med 2019;10(3): 394-7 Corresponding Author: Ebru Kuyumcu Savan, Faculty of Pharmacy, Analytical Chemistry Division, İnönü University, Malatya, Turkey. T.: +90 4223411216 F.: +90 4223411217 E-Mail: ebru.savan@inonu.edu.tr ORCID ID: 0000-0002-8851-0907 Abstract Aim: In this study, it was aimed to determine the reduced Glutathione (GSH) at the modified sensor with a simple and reliable electroanalytical method. Material and Method: Cyclic voltammetry and differential pulse voltammetry techniques were used for the determination of reduced-GSH. And the modified sensor was fabricated by dropping single-walled carbon nanotube (SWCNT) dispersion onto the surface of the glassy carbon electrode. Results: The determination of reduced-GSH was accomplished at SWCNT modified sensor. The relationship between the current responses of the sensor and the concentrations of the reduced-GSH (1.0 x 10-8 5.0 x 10-8 M) showed excellent linearity and the detection limit was calculated as 75 nM. Discussion: Determination of trace amounts of reduced-GSH in urine samples was successfully applied at the modified sensor. The results showed that modification of the glassy carbon electrode with SWCNT could provide a new strategy for determining the concentration of GSH in physiological solutions.

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生物样品中还原型谷胱甘肽的电分析测定

DOI:10.4328/JCAM.6155接收日期：2019年1月7日接受日期：2019年底1月14日在线发布日期：2019年初1日印刷日期：2019年月1日《临床分析医学杂志》；10（3）：394-7通讯作者：Ebru Kuyumcu Savan，土耳其马拉蒂亚伊恩努大学分析化学系药学院。电话：+90 4223411216传真：+90 422 3411217电子邮箱：ebru.savan@inonu.edu.trORCID ID:0000-0002-8851-0907摘要目的：本研究旨在用一种简单可靠的电分析方法测定改良传感器中还原的谷胱甘肽（GSH）。材料与方法：采用循环伏安法和微分脉冲伏安法测定还原态谷胱甘肽。通过将单壁碳纳米管（SWCNT）分散体滴加到玻碳电极表面，制备了改进的传感器。结果：在SWCNT修饰的传感器上完成了还原GSH的测定。传感器的电流响应与还原的GSH浓度（1.0 x 10-8 5.0 x 10-8M）之间的关系显示出极好的线性，检测限计算为75nM。讨论：在改进的传感器上成功地应用了尿样品中微量还原GSH的测定。结果表明，SWCNT修饰玻碳电极可以为测定生理溶液中GSH的浓度提供一种新的策略。

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Journal of Clinical and Analytical Medicine MEDICINE, GENERAL & INTERNAL-

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期刊介绍： The Annals of Clinical and Analytical Medicine is an international open-access journal containing peer-reviewed high-quality articles on clinical medicine in the areas of all research study types, reviews, and case reports. Our journal has become an important platform with the help of language support services, which make it easier for writers who have English as their second language to share their clinical experiences with the world.