GC-MS Profiling and Pharmacological Potential of Physconia venusta (Ach.) Poelt.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-07-12 DOI:10.4274/tjps.galenos.2023.91126

Ibtissem Zeghina, Ibtissem El Ouar, Maya Abir Tartouga, Mohamed Badreddine Mokhtari, Daniel Elieh-Ali-Komi, Lynda Gali, Chawki Bensouici

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引用次数: 0

Abstract

Objectives: Lichens are complex symbiotic organisms that generate various bioactive compounds with significant therapeutic value. We investigated the chemical composition and bioactivity of the acetone extract of the Algerian lichen Physconia venusta (Ach.) poet.

Materials and methods: Phytochemical screening was performed using gas chromatography-mass spectrometry (GC-MS). The antibacterial activity was assessed against Escherichia coli, Pseudomonas aeruginosa, Salmonella enteritidis, Salmonella typhi, Staphylococcus aureus, Listeria monocytogenes, and Bacillus subtilis using an agar diffusion test with the determination of the minimal inhibition concentration (MIC), while the antioxidant activity was determined using different chemical methods (DPPH, ABTS, CUPRAC, reducing power, superoxide anion scavenging, β-carotene bleaching, and metal chelate). In addition, cytotoxic activity was tested using Artemia salina (Brine shrimp) bioassay.

Results: The studied extract exhibited intense antibacterial activity against E. coli and S. aureus with inhibition diameters of 28 ± 0.01 and 22 ± 0.01 mm, respectively, with a MIC value of 6.25 mg/mL and a selectivity index of 2.8. The obtained extract showed different antioxidant trends depending on the selected assay. GC-MS analysis revealed many secondary metabolites.

Conclusion: P. venusta, a type of lichen, is a potential source of bioactive substances that could be used in pharmaceuticals.

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金星藻（Physconia venusta）Poelt的气相色谱-质谱分析及其药理潜力

目的：地衣是一种复杂的共生生物，能产生各种具有重要治疗价值的生物活性化合物。我们试图研究阿尔及利亚地衣Physconia venusta（Ach.）Poelt的丙酮提取物的化学成分和生物活性。材料与方法：采用气相色谱-质谱联用技术进行植物化学筛选。使用琼脂扩散试验测定最小抑制浓度（MIC），评估对大肠杆菌、铜绿假单胞菌、肠炎沙门氏菌、斑疹伤寒杆菌、金黄色葡萄球菌、李斯特菌和枯草芽孢杆菌的抗菌活性，同时使用不同的化学方法（DPPH、ABTS、CURAC、还原力、超氧化物阴离子清除、β-胡萝卜素漂白和金属螯合物）测定抗氧化活性。此外，使用卤虫（盐水虾）生物测定法测试细胞毒性活性。结果：该提取物对大肠杆菌和金黄色葡萄球菌具有较强的抗菌活性，抑菌直径分别为28±0.01和22±0.01mm，MIC值为6.25mg/ml，选择性指数为2.8。所获得的提取物根据所选择的测定显示出不同的抗氧化趋势。GC-MS分析显示存在许多次级代谢产物。结论：金星苔草是一种潜在的药用生物活性物质来源。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.