A preliminary study of Salmonella spp., Listeria monocytogenes, Escherichia coli O157, Enterococcus faecalis and Clostridium spp. in Irish cattle

IF 0.9 4区农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY

Irish Journal of Agricultural and Food Research Pub Date : 2021-06-03 DOI:10.15212/IJAFR-2020-0122

L. Russell, C. Galindo, P. Whyte, D. Bolton

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引用次数: 1

Abstract

Although Salmonella spp., Escherichia coli O157, Listeria monocytogenes, Enterococcus faecalis and Clostridium spp. present a significant food safety and/or spoilage issue for the beef sector, there are limited data on their prevalence in Irish cattle. The objectives of this preliminary study were to investigate the distribution (percentage of farms positive) of Salmonella spp., E. coli O157, L. monocytogenes, E. faecalis and Clostridium spp. and the overall prevalence (%) of these bacteria in cattle on a small cohort of Irish beef farms. A total of 121 fresh bovine faecal samples were obtained on 10 randomly selected beef farms in the Northeast of Ireland and tested for the target pathogens using standard culture-based methods. Presumptive positives were confirmed using previously published polymerase chain reaction (PCR) methods. Salmonella were not detected in any of the samples. E. coli O157, L. monocytogenes, E. faecalis and Clostridium spp. were present on 50%, 40%, 100% and 100% of farms, respectively, with overall (all farms) prevalence rates in cattle of 9%, 8.2%, 61.9% and 87.6%, respectively. This study suggests that E. coli O157 may be more prevalent than previously thought and L. monocytogenes, E. faecalis and Clostridium spp. are widespread in Irish beef animals.

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爱尔兰牛中沙门氏菌、单核细胞增生性李斯特菌、O157大肠杆菌、粪肠球菌和梭菌的初步研究

尽管沙门氏菌属、O157大肠杆菌、单核细胞增多性李斯特菌、粪肠球菌和梭菌属对牛肉行业来说是一个重大的食品安全和/或腐败问题，但关于它们在爱尔兰牛中的流行率的数据有限。这项初步研究的目的是调查沙门氏菌、O157大肠杆菌、单核细胞增多性李斯特菌、粪大肠杆菌和梭菌的分布（农场阳性率）。以及这些细菌在爱尔兰牛肉农场的牛中的总体流行率（%）。在爱尔兰东北部随机选择的10个牛肉农场共获得121份新鲜牛粪便样本，并使用基于标准培养的方法检测目标病原体。使用先前发表的聚合酶链式反应（PCR）方法确认推定阳性。在任何样本中均未检测到沙门氏菌。O157大肠杆菌、单核细胞增多性李斯特菌、粪大肠杆菌和梭菌属分别存在于50%、40%、100%和100%的农场，牛的总体（所有农场）患病率分别为9%、8.2%、61.9%和87.6%。这项研究表明，O157大肠杆菌可能比以前认为的更普遍，单核细胞增多性李斯特菌、粪大肠杆菌和梭菌在爱尔兰牛肉动物中广泛存在。

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来源期刊

Irish Journal of Agricultural and Food Research 农林科学-农业综合

CiteScore

2.50

自引率

20.00%

发文量

审稿时长

>36 weeks

期刊介绍： The Irish Journal of Agricultural and Food Research is a peer reviewed open access scientific journal published by Teagasc (Agriculture and Food Development Authority, Ireland). Manuscripts on any aspect of research of direct relevance to Irish agriculture and food production, including plant and animal sciences, food science, agri environmental science, soils, engineering, buildings, economics and sociology, will be considered for publication. The work must demonstrate novelty and relevance to the field of research. Papers published or offered for publication elsewhere will not be considered, but the publication of an abstract does not preclude the publication of the full paper in this journal.