Features of the Structure and Electrophysiological Properties of a Novel Porin from the Marine Bacterium Marinomonas primoryensis

Abstract

Using the method of protein reconstitution into planar bilayer lipid membranes, the electrophysiological properties of a novel porin channel from the marine bacterium Marinomonas primoryensis (MpOmp) were characterized. The main characteristics were determined: the conductivity value of the single MpOmp channel, its selectivity, and the values of the critical closing potential in various media (neutral, weakly acidic, alkaline). Using an in silico approach, the geometric characteristics of the MpOmp pore and the distribution of charges at the mouth and inside the porin channel were predicted.