Salidroside attenuates oxygen and glucose deprivation-induced neuronal injury by inhibiting ferroptosis

IF 1.7 4区医学 Q3 TROPICAL MEDICINE

Asian Pacific journal of tropical biomedicine Pub Date : 2023-02-01 DOI:10.4103/2221-1691.369611

Ying-Zhi Li, Ai-ping Wu, Dan-dan Wang, Pan-Pan Yang, Bin Sheng

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引用次数: 1

Abstract

Objective: To evaluate the effect of salidroside on oxygen and glucose deprivation (OGD)-treated NT2 cells and its underlying mechanisms of action. Methods: Retinoic acid was used to induce the differentiation of NT2 cells into neurons. The effects of salidroside on survival, apoptosis, inflammatory response, and oxidative stress of neurons undergoing OGD were evaluated. Using precursor cells as controls, the effect of salidroside on the differentiation progression of OGD-treated cells was evaluated. In addition, the effect of erastin, a ferroptosis inducer, on NT2 cells was examined to investigate the underlying mechanisms of neuroprotective action of salidroside. Results: Salidroside alleviated the effects of OGD on neuronal survival, apoptosis, inflammation, and oxidative stress, and promoted NT2 cell differentiation. Moreover, salidroside prevented ferroptosis of OGD-treated cells, which was abolished following erastin treatment, indicating that ferroptosis mediated the regulatory pathway of salidroside. Conclusions: Salidroside attenuates OGD-induced neuronal injury by inhibiting ferroptosis and promotes neuronal differentiation.

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红景天苷通过抑制脱铁性贫血减轻缺氧和缺糖诱导的神经元损伤

目的：评价红景天苷对缺氧缺糖（OGD）处理的NT2细胞的影响及其作用机制。方法：用维甲酸诱导NT2细胞向神经元分化。评价了红景天苷对OGD神经元存活、细胞凋亡、炎症反应和氧化应激的影响。使用前体细胞作为对照，评估了红景天苷对OGD处理的细胞分化进程的影响。此外，还检测了脱铁诱导剂erastin对NT2细胞的影响，以研究红景天苷神经保护作用的潜在机制。结果：红景天苷减轻了OGD对神经元存活、凋亡、炎症和氧化应激的影响，并促进了NT2细胞的分化。此外，红景天苷预防了OGD处理的细胞的脱铁性贫血，该现象在erastin处理后被消除，这表明脱铁性腹泻介导了红景天苷的调节途径。结论：红景天苷通过抑制脱铁性贫血减轻OGD诱导的神经元损伤，并促进神经元分化。

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来源期刊

Asian Pacific journal of tropical biomedicine Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (miscellaneous)

CiteScore

3.10

自引率

11.80%

发文量

2056

审稿时长

4 weeks

期刊介绍： The journal will cover technical and clinical studies related to health, ethical and social issues in field of biology, bacteriology, biochemistry, biotechnology, cell biology, environmental biology, microbiology, medical microbiology, pharmacology, physiology, pathology, immunology, virology, toxicology, epidemiology, vaccinology, hematology, histopathology, cytology, genetics and tropical agriculture. Articles with clinical interest and implications will be given preference.