Qualitative Analysis of Drug-Containing Plasma and its Application to Quantitative Analysis and Pharmacokinetic Study of Zexie Decoction Using UPLC-MS/MS

IF 3.8 3区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Frontiers in Chemistry Pub Date : 2022-02-22 DOI:10.3389/fchem.2022.815886

Jiashuo Wu, Shunliang Zheng, Fangqing Zhang, Haonan Ruan, Haotian Xue, Jing-Xun Wang, Zhuang-Zhuang Li, Wei-Yi Jin, Weihua Wang, Jing Xia, Yue Shi

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引用次数: 2

Abstract

ZeXie Decoction (ZXD) is one of the traditional Chinese medicine formulas (TCMFs) comprising Alisma orientalis (Sam.) Juzep. (ZX) and Atractylodes macrocephala Koidz. (BZ) in 5:2 ratios and is widely employed in clinical applications since ancient times. In this study, UHPLC-QE-Orbitrap-MS was used for qualitative analysis of ZXD in rats’ plasma after a single oral dose of 750 mg/kg body weight. Afterward, UHPLC-Q-TRAP-MS/MS was used for simultaneous analysis of three bioactive chemical compounds including alisol A, alisol B, and alisol A 24-acetate in ZXD’s ethanol extract. Subsequently, the pharmacokinetic profiles of the three analytes were investigated in rat plasma utilizing UHPLC-Q-TRAP-MS/MS. The multiple reaction monitoring (MRM) mode for the three analytes were at m/z 508.4→383.2 for alisol A, m/z 490.4→365.2 for alisol B, and m/z 550.4→515.5 for alisol A 24-acetate. The analysis method was validated in terms of its accuracy, stability, repeatability, linearity, spiked recovery and matrix effect. As a result, twenty-five chemical constituents of ZXD were putatively identified in plasma, and rapid, sensitive, and accurate methods were established for the quantitative analysis and pharmacokinetic study of ZXD. The findings of this study can provide a scientific base for further study of in vivo pharmacokinetics of TCMFs.

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UPLC-MS/MS法定性分析泽泻汤血浆及其药代动力学研究

泽泻汤是以泽泻为主要成分的中药方剂之一。（ZX）和白术。（BZ）的比例为5:2，自古以来就广泛应用于临床。在本研究中，UHPLC QE Orbitrap MS用于大鼠单次口服750 mg/kg体重后血浆中ZXD的定性分析。然后，使用UHPLC-Q-TRAP-MS/MS同时分析ZXD乙醇提取物中的三种生物活性化合物，包括alisol A、alisol B和alisol A24乙酸酯。随后，利用UHPLC-Q-TRAP-MS/MS研究了三种分析物在大鼠血浆中的药代动力学特征。三种分析物的多重反应监测（MRM）模式为m/z 508.4→383.2阿利索尔A，m/z 490.4→阿利索尔B为365.2，m/z为550.4→515.5，对于alisol A 24乙酸酯。从准确度、稳定性、重复性、线性、加标回收率和基质效应等方面对该分析方法进行了验证。结果，在血浆中鉴定出25种ZXD化学成分，建立了快速、灵敏、准确的ZXD定量分析和药代动力学研究方法。本研究结果可为进一步研究中药复方制剂的体内药代动力学提供科学依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Chemistry Chemistry-General Chemistry

CiteScore

8.50

自引率

3.60%

发文量

1540

审稿时长

12 weeks

期刊介绍： Frontiers in Chemistry is a high visiblity and quality journal, publishing rigorously peer-reviewed research across the chemical sciences. Field Chief Editor Steve Suib at the University of Connecticut is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to academics, industry leaders and the public worldwide. Chemistry is a branch of science that is linked to all other main fields of research. The omnipresence of Chemistry is apparent in our everyday lives from the electronic devices that we all use to communicate, to foods we eat, to our health and well-being, to the different forms of energy that we use. While there are many subtopics and specialties of Chemistry, the fundamental link in all these areas is how atoms, ions, and molecules come together and come apart in what some have come to call the “dance of life”. All specialty sections of Frontiers in Chemistry are open-access with the goal of publishing outstanding research publications, review articles, commentaries, and ideas about various aspects of Chemistry. The past forms of publication often have specific subdisciplines, most commonly of analytical, inorganic, organic and physical chemistries, but these days those lines and boxes are quite blurry and the silos of those disciplines appear to be eroding. Chemistry is important to both fundamental and applied areas of research and manufacturing, and indeed the outlines of academic versus industrial research are also often artificial. Collaborative research across all specialty areas of Chemistry is highly encouraged and supported as we move forward. These are exciting times and the field of Chemistry is an important and significant contributor to our collective knowledge.