Dissolution and bioactivity of a sol-gel derived borate glass in six different solution media

Q1 Materials Science

Biomedical Glasses Pub Date : 2019-01-01 DOI:10.1515/bglass-2019-0009

William C. Lepry, E. Rezabeigi, Sophia Smith, S. Nazhat

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引用次数: 11

Abstract

Abstract Sol-gel derived bioactive borate glasses (SGBGs) rapidly convert to hydroxycarbonated apatite (HCA) in simulated body fluid (SBF), in vitro. While previous studies have examined the influence of processing and composition on bioactivity, the effect of the in vitro dissolution media has not been well examined for these glasses. In this study, the mineral conversion of a SGBG substituted 45S5 Bioglass® formulation (“B46”, (46.1)B2O3-(26.9)CaO-(24.4)Na2O-(2.6)P2O5, mol%), was examined in six different dissolution media: SBF, tris(hydroxymethyl)aminomethane (TRIS, pH 7.4) buffer, Dulbecco’s phosphate buffered saline (PBS, 1X), Dulbecco’s Modified Eagle Medium (DMEM, 1X), 0.9% Saline (SAL), and deionized water (DIW) at 1.5 mg/mL for 10 min, 2h, and 1d. All media underwent a rapid increase in pH as a result of glass dissolution and ion release. B46 in SBF, TRIS, and PBS converted to HCA while B46 in DMEM, SAL, and DIW converted to calcite according to attenuated total reflectance-Fourier-transform infrared spectroscopy, X-ray diffraction, and scanning electron microscopy. The ratio of B46 to SBF was also examined at 3, 6, 12, and 24 mg/mL for 1d. These results help elucidate the dissolution and mineral conversion of borate glasses and help provide insights into optimizing pre-conditioning treatments for both in vitro and in vivo analyses.

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溶胶-凝胶衍生硼酸盐玻璃在六种不同溶液介质中的溶解性和生物活性

摘要溶胶-凝胶衍生的生物活性硼酸盐玻璃（SGBG）在体外模拟体液（SBF）中快速转化为羟基碳酸磷灰石（HCA）。虽然先前的研究已经检验了加工和组成对生物活性的影响，但体外溶解介质对这些玻璃的影响还没有得到很好的检验。在本研究中，SGBG取代的45S5 Bioglass®制剂（“B46”，（46.1）B2O3-（26.9）CaO-（24.4）Na2O-（2.6）P2O5，mol%）的矿物转化率在六种不同的溶解介质中进行了检测：SBF、三（羟甲基）氨基甲烷（tris，pH 7.4）缓冲液、Dulbecco磷酸盐缓冲盐水（PBS，1X）、Dulbecko改良鹰培养基（DMEM，1X）和0.9%盐水（SAL），和1.5mg/mL的去离子水（DIW），持续10分钟、2小时和1天。由于玻璃溶解和离子释放，所有介质的pH值都迅速增加。根据衰减全反射傅立叶变换红外光谱、X射线衍射和扫描电子显微镜，SBF、TRIS和PBS中的B46转化为HCA，而DMEM、SAL和DIW中的B46。B46与SBF的比例也在3、6、12和24mg/mL下检测1d。这些结果有助于阐明硼酸盐玻璃的溶解和矿物转化，并有助于为优化体外和体内分析的预处理提供见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomedical Glasses Materials Science-Surfaces, Coatings and Films

自引率

0.00%

发文量

审稿时长

17 weeks

期刊介绍： Biomedical Glasses is an international Open Access-only journal covering the field of glasses for biomedical applications. The scope of the journal covers the science and technology of glasses and glass-based materials intended for applications in medicine and dentistry. It includes: Chemistry, physics, structure, design and characterization of biomedical glasses Surface science and interactions of biomedical glasses with aqueous and biological media Modeling structure and reactivity of biomedical glasses and their interfaces Biocompatibility of biomedical glasses Processing of biomedical glasses to achieve specific forms and functionality Biomedical glass coatings and composites In vitro and in vivo evaluation of biomedical glasses Glasses and glass-ceramics in engineered regeneration of tissues and organs Glass-based devices for medical and dental applications Application of glasses and glass-ceramics in healthcare.