Preparation, characterization, and formation mechanism of different biological calcium carbonate (CaCO3) induced by Bacillus mucilaginosus and Bacillus alcalophilus
{"title":"Preparation, characterization, and formation mechanism of different biological calcium carbonate (CaCO3) induced by Bacillus mucilaginosus and Bacillus alcalophilus","authors":"Tianwen Zheng, Daibing Hou, Wenpeng Leng, Peizhong Li, Wenxia Wei","doi":"10.1007/s11051-023-05833-z","DOIUrl":null,"url":null,"abstract":"<div><p>Microbial-induced calcium carbonate precipitation (MICP) is a common mineralization phenomenon in nature, which has the advantages of green and environmental protection. In this paper, <i>Bacillus mucilaginosu</i>s and <i>Bacillus alcalophilus</i> were selected to study the dynamic process of MICP in alkaline solution through the changes of Ca<sup>2+</sup> concentration, pH value, calcification rate, and Zeta potential, and then the control factors affecting the generation of biological CaCO<sub>3</sub> were revealed. Subsequently, various characterization methods were used to explore the effects of different microbial species on the morphology, crystal polymorph, crystalline size, reflectivity, specific surface area, pore volume, and porosity of biological CaCO<sub>3</sub>, and the formation mechanism of biological CaCO<sub>3</sub> was also analyzed carefully. The experimental results showed that microbial mineralization was the control step affecting the formation of biological CaCO<sub>3</sub>. Due to the different microbial mineralization mechanisms, the properties of biological CaCO<sub>3</sub> were also different. Compared with the reference CaCO<sub>3</sub>, the CaCO<sub>3</sub> induced by <i>Bacillus mucilaginosu</i>s was mainly calcite with uniformly dispersed oblique hexahedron shape, while the CaCO<sub>3</sub> induced by <i>Bacillus alcalophilus</i> was mainly vaterite with uniform spherical shape. Meanwhile, the mechanism of MICP showed that <i>Bacillus mucilaginosus</i> mainly promoted the production of biological CaCO<sub>3</sub> through the microbial enzymatic action (carbonic anhydrase), while <i>Bacillus alcalophilus</i> mainly controlled CaCO<sub>3</sub> precipitation through the microbial metabolic decomposition. Generally, the paper reveals the diversity of biomineralization by studying the properties and mechanisms of CaCO<sub>3</sub> induced by different microbial species, which provides the theoretical basis for the application of MICP.</p></div>","PeriodicalId":653,"journal":{"name":"Journal of Nanoparticle Research","volume":"25 9","pages":""},"PeriodicalIF":2.1000,"publicationDate":"2023-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Nanoparticle Research","FirstCategoryId":"88","ListUrlMain":"https://link.springer.com/article/10.1007/s11051-023-05833-z","RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
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Abstract
Microbial-induced calcium carbonate precipitation (MICP) is a common mineralization phenomenon in nature, which has the advantages of green and environmental protection. In this paper, Bacillus mucilaginosus and Bacillus alcalophilus were selected to study the dynamic process of MICP in alkaline solution through the changes of Ca2+ concentration, pH value, calcification rate, and Zeta potential, and then the control factors affecting the generation of biological CaCO3 were revealed. Subsequently, various characterization methods were used to explore the effects of different microbial species on the morphology, crystal polymorph, crystalline size, reflectivity, specific surface area, pore volume, and porosity of biological CaCO3, and the formation mechanism of biological CaCO3 was also analyzed carefully. The experimental results showed that microbial mineralization was the control step affecting the formation of biological CaCO3. Due to the different microbial mineralization mechanisms, the properties of biological CaCO3 were also different. Compared with the reference CaCO3, the CaCO3 induced by Bacillus mucilaginosus was mainly calcite with uniformly dispersed oblique hexahedron shape, while the CaCO3 induced by Bacillus alcalophilus was mainly vaterite with uniform spherical shape. Meanwhile, the mechanism of MICP showed that Bacillus mucilaginosus mainly promoted the production of biological CaCO3 through the microbial enzymatic action (carbonic anhydrase), while Bacillus alcalophilus mainly controlled CaCO3 precipitation through the microbial metabolic decomposition. Generally, the paper reveals the diversity of biomineralization by studying the properties and mechanisms of CaCO3 induced by different microbial species, which provides the theoretical basis for the application of MICP.
期刊介绍：
The objective of the Journal of Nanoparticle Research is to disseminate knowledge of the physical, chemical and biological phenomena and processes in structures that have at least one lengthscale ranging from molecular to approximately 100 nm (or submicron in some situations), and exhibit improved and novel properties that are a direct result of their small size.
Nanoparticle research is a key component of nanoscience, nanoengineering and nanotechnology.
The focus of the Journal is on the specific concepts, properties, phenomena, and processes related to particles, tubes, layers, macromolecules, clusters and other finite structures of the nanoscale size range. Synthesis, assembly, transport, reactivity, and stability of such structures are considered. Development of in-situ and ex-situ instrumentation for characterization of nanoparticles and their interfaces should be based on new principles for probing properties and phenomena not well understood at the nanometer scale. Modeling and simulation may include atom-based quantum mechanics; molecular dynamics; single-particle, multi-body and continuum based models; fractals; other methods suitable for modeling particle synthesis, assembling and interaction processes. Realization and application of systems, structures and devices with novel functions obtained via precursor nanoparticles is emphasized. Approaches may include gas-, liquid-, solid-, and vacuum-based processes, size reduction, chemical- and bio-self assembly. Contributions include utilization of nanoparticle systems for enhancing a phenomenon or process and particle assembling into hierarchical structures, as well as formulation and the administration of drugs. Synergistic approaches originating from different disciplines and technologies, and interaction between the research providers and users in this field, are encouraged.
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