Microgreen Variety Impacts Leaf Surface Persistence of a Human Norovirus Surrogate

IF 4.1 2区农林科学 Q2 ENVIRONMENTAL SCIENCES

Food and Environmental Virology Pub Date : 2022-09-23 DOI:10.1007/s12560-022-09536-x

Wenjun Deng, Kristen E. Gibson

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Abstract

Human norovirus (HuNoV) is a pathogenic agent that is frequently associated with foodborne disease outbreaks linked to fresh produce. Within microgreen production systems, understanding of virus transmission routes and persistence is limited. To investigate virus persistence on microgreen leaf surfaces, this study mimicked virus contaminations caused during microgreen handling by farm workers or during overhead irrigation with contaminated water. Specifically, approximately 5 log PFU of Tulane virus (TV)—a HuNoV surrogate—was inoculated on sunflower (SF) and pea shoot (PS) microgreen leaves at 7-day age. The virus reduction on SF was significantly higher than PS (p < 0.05). On day 10, total TV reduction for SF and PS were 3.70 ± 0.10 and 2.52 ± 0.30 log PFU/plant, respectively. Under the environmental scanning electron microscope (ESEM) observation, the leaf surfaces of SF were visually smoother than PS, while their specific effect on virus persistence were not further characterized. Overall, this study revealed that TV persistence on microgreen leaves was plant variety dependent. In addition, this study provided a preliminary estimation on the risk of HuNoV contamination in a microgreen production system which will aim the future development of prevention and control measures.

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微绿变种对人类诺如病毒替代物叶片表面持久性的影响

人诺如病毒(HuNoV)是一种病原体，经常与与新鲜农产品有关的食源性疾病暴发有关。在微绿生产系统中，对病毒传播途径和持久性的了解是有限的。为了研究病毒在微绿叶片表面的持久性，本研究模拟了农场工人处理微绿或用污染的水进行架空灌溉时造成的病毒污染。具体地说，在7日龄的向日葵(SF)和豌豆芽(PS)的微绿叶上接种了大约5 log PFU的杜兰病毒(TV)——HuNoV的替代物。SF上的病毒减毒量显著高于PS (p < 0.05)。在第10天，SF和PS的总TV减少量分别为3.70±0.10和2.52±0.30 log PFU/株。在环境扫描电镜(ESEM)下观察，SF的叶片表面比PS更光滑，但其对病毒持久性的特异性影响尚未进一步表征。总体而言，本研究揭示了植物微绿叶片上的TV持久性具有品种依赖性。此外，本研究还对微绿生产系统中HuNoV污染的风险进行了初步估计，为今后防治措施的制定提供了依据。

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来源期刊

Food and Environmental Virology ENVIRONMENTAL SCIENCES-MICROBIOLOGY

CiteScore

6.50

自引率

2.90%

发文量

审稿时长

1 months

期刊介绍： Food and Environmental Virology publishes original articles, notes and review articles on any aspect relating to the transmission of pathogenic viruses via the environment (water, air, soil etc.) and foods. This includes epidemiological studies, identification of novel or emerging pathogens, methods of analysis or characterisation, studies on survival and elimination, and development of procedural controls for industrial processes, e.g. HACCP plans. The journal will cover all aspects of this important area, and encompass studies on any human, animal, and plant pathogenic virus which is capable of transmission via the environment or food.