Control of magnetic susceptibility of probiotic strain Lactobacillus rhamnosus GG

Abstract

The paper investigates the increase in the natural magnetically controlled properties of probiotic microorganisms Lactobacillus rhamnosus GG (LGG) and their ability to form magnetically sensitive inclusions (MsI). The magnetic susceptibility of LGG was increased by modifying the nutrient medium composition and by cultivating the probiotic culture in a constant magnetic field. Therefore, this study can be useful for their further use as magnetically controlled vectors since it is being extensively researched for their use in targeted drug delivery in cancer treatment, for the prevention of chemotherapy complications, etc. The results of this study indicate that growing microorganisms with natural magnetically-guided properties in a modified medium with iron chelate and in an external magnetic field leads to an increase in the magnetic susceptibility of LGG by 1.8 and 2.6 times, respectively, compared with the control. The best magnetic susceptibility was recorded for LGG suspensions, which were grown in a constant magnetic field and cultured in modified medium with iron chelate. The LGG suspension, grown both in a constant magnetic field and on a modified medium with iron chelate, had the highest magnetic susceptibility, and it was 4.8 times larger than the magnetic susceptibility in the control.