Microbial Infection of Orthodontic Synthetic Intermaxillary Elastics in Differents Types of Manipulation: An In Vitro Study

Flvio de Mendona Copello, D. Alviano, M. M. Souza, A. Bolognese, Angela Rita Pontes Azevedo
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Abstract

Background: Intermaxillary elastic is considered important sources of energy in orthodontic movement. However, the majority of this type of material, mainly those of Brazilian brands, is sold in packets containing 1000 units, which obliges the orthodontist or assistant to manipulate these, in order to separate them into a smaller quantity for each patient. The care taken by the working team is essential for preserving the integrity of the health of both the team and patients. Aim/objectives: the purpose of this study was to perform an in vitro evaluation of synthetic intermaxillary elastics from different manufacturers, to verify the presence of microorganisms at the time of opening the package and its application, by different forms of manipulation. Methods: 120 synthetic intermaxillary elastics were divided into four groups according to different trademarks and type of manipulation. These were incubated at 37 o C and evaluated after time intervals of 24 hours, 48 hours, 72 hours, 4 days, 7 days and 10 days. The cultures were analyzed by visual inspection and when the culture medium presented turbidity, the presence of contamination was considered. Findings/results: Bacterial contamination was only found in the groups that occurred hand manipulation of the materials. Discussion: The elastics tested in this study presented no evidence of microbial contamination before manipulation; manipulation of the elastics with the use of gloves and sterile forceps prevented contamination by microorganisms; the elastic manipulation with hands is not recommended.
不同手法对正畸合成上颌间弹性材料微生物感染的体外研究
背景:颌间弹性被认为是正畸运动中重要的能量来源。然而,大多数此类材料,主要是巴西品牌的材料,都是以装有1000个单位的包装出售的,这迫使正畸医生或助理对这些材料进行操作,以便为每个患者将其分成更小的数量。工作团队所采取的护理对于维护团队和患者健康的完整性至关重要。目的/目的:本研究的目的是对不同制造商的合成颌间弹性体进行体外评估,以通过不同形式的操作验证打开包装时是否存在微生物及其应用。方法:根据不同商标和手法类型,将120种合成颌间弹力带分为四组。这些细胞在37℃下孵育,并在24小时、48小时、72小时、4天、7天和10天的时间间隔后进行评估。通过目视检查对培养物进行分析,当培养基呈现浊度时,考虑污染的存在。发现/结果:细菌污染仅在手动操作材料的组中发现。讨论:本研究中测试的弹性体在操作前没有微生物污染的证据;使用手套和无菌钳子操作松紧带,防止微生物污染;不建议用手进行弹性操作。
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