The Effect of Malathion Concentration and Exposure Time on Histopathological Changes in the Liver and Gill of Rainbow Trout

IF 1.9 4区 农林科学 Q2 FISHERIES
Hamed Ghafarifarsani, M. Raeeszadeh, S. Hajirezaee, Sadegh Ghafari Farsani, Mohammad Mansouri Chorehi
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Abstract

Exposure of aquatic organisms to organophosphorus pollutants is a subject of keen interest to biologists and environmental scientists. Examining histopathological changes in the tissues of exposed animals can provide great insights to understand the health condition of the organisms. This study examined the effects of malathion concentration and exposure time on the liver and gill tissues of the rainbow trout (Oncorhynchus mykiss) in a laboratory condition and tried to provide a quantitative assessment for the analysis of these effects. The experiment was conducted in three treatments including 0.025, 0.05, and 0.075 mg/L of malathion for 1, 5, and 9 days with a nonexposed group as control, in three replicates. The liver and gill samples were fixed in buffered formalin. About 5 µ tissue sections were prepared using the conventional histological methods and stained using the hematoxylin–eosin method. Histopathological changes in the liver and gill tissues were quantified by grading and the resulting data were analyzed by rank-based estimation. The results showed that histopathological changes in the liver and gill tissues are more affected by the malathion concentration than by the duration of the exposure. However, longer exposure had an intensifying effect on the tissue damage caused by the malathion at higher concentrations. The presence of melanomacrophages as an indicator of malathion toxicity was determined. The fish exposed to 0.075 mg/L malathion for 9 days showed atrophy in the liver and gill tissues, indicating cell death and functional inactivation. Histopathological changes in the liver and gills confirmed the dose-dependent effect of malathion on the rainbow trout.
马拉硫磷浓度和暴露时间对虹鳟鱼肝脏和鳃组织病理变化的影响
水生生物暴露于有机磷污染物是生物学家和环境科学家非常感兴趣的课题。检查暴露动物组织的组织病理学变化可以为了解生物体的健康状况提供很好的见解。本研究在实验室条件下检测了马拉硫磷浓度和暴露时间对虹鳟(Oncorhynchus mykiss)肝脏和鳃组织的影响,并试图为这些影响的分析提供定量评估。实验分三个处理进行,包括0.025、0.05和0.075 mg/L马拉硫磷,持续1、5和9天,以非暴露组为对照,三次重复。将肝脏和鳃样品固定在缓冲福尔马林中。约5 使用传统组织学方法制备µ组织切片,并使用苏木精-伊红法染色。通过分级对肝脏和鳃组织的组织病理学变化进行量化,并通过基于秩的估计对所得数据进行分析。结果表明,肝脏和鳃组织的组织病理学变化更受马拉硫磷浓度的影响,而不是受暴露时间的影响。然而,暴露时间越长,马拉硫磷浓度越高,对组织损伤的影响越大。确定了作为马拉硫磷毒性指标的黑巨噬细胞的存在。鱼暴露在0.075 mg/L马拉硫磷作用9天,肝和鳃组织出现萎缩,表明细胞死亡和功能失活。肝脏和鳃的组织病理学变化证实了马拉硫磷对虹鳟鱼的剂量依赖性作用。
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来源期刊
Aquaculture Research
Aquaculture Research 农林科学-渔业
CiteScore
4.60
自引率
5.00%
发文量
464
审稿时长
5.3 months
期刊介绍: International in perspective, Aquaculture Research is published 12 times a year and specifically addresses research and reference needs of all working and studying within the many varied areas of aquaculture. The Journal regularly publishes papers on applied or scientific research relevant to freshwater, brackish, and marine aquaculture. It covers all aquatic organisms, floristic and faunistic, related directly or indirectly to human consumption. The journal also includes review articles, short communications and technical papers. Young scientists are particularly encouraged to submit short communications based on their own research.
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