In Vitro Micropropagation of Andrographis macrobotrys

IF 1.2 Q4 PHARMACOLOGY & PHARMACY

Journal of Herbs, Spices and Medicinal Plants Pub Date : 2021-08-19 DOI:10.1080/10496475.2021.1964012

S. S. Kadapatti, H. N. Murthy

引用次数: 4

Abstract

ABSTRACT In vitro propagation method was developed for Andrographis macrobotrys for large-scale multiplication. Nodal explants were cultured on Murashige and Skoog (MS) medium containing cytokinins including 6-benzyl amino purine (BAP), kinetin (KN), 2-isopentyl adenine (2-iP) and thidiazuron (TDZ) at 2.5, 5, 7.5 or 10 μM L−1. Multiple axillary shoots (32 shoots per explants) were regenerated from nodal explants on MS medium supplemented with 7.5 μM L−1 BAP. Quarter strength MS medium supplemented with 5 μM L−1 indole-3-butyric acid (IBA) was effective for induction of roots from the regenerated shoots and on this medium optimal of 49 roots were regenerated from shoots. Micropropagated plants were transplanted to pots containing soil and coco-peat (1:1) mix and maintained in growth chamber at 25 ± 2°C, relative humidity 80%, irradiance of 100 µmol m−2 s−1 for 16-h photoperiod. After eight weeks, plants were transferred to field where soil was mixed with farmyard manure and sand.

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大穿心莲的体外微繁研究

摘要建立了穿心莲大型繁殖的体外繁殖方法。在含有细胞分裂素的Murashige和Skoog（MS）培养基上以2.5、5、7.5或10μM L−1培养节外植体，细胞分裂剂包括6-苄基氨基嘌呤（BAP）、激动素（KN）、2-异戊基腺嘌呤（2-iP）和噻二唑仑（TDZ）。在添加7.5μM L−1 BAP的MS培养基上，从节外植体再生出多个腋芽（每个外植体32个芽）。添加5μM L−1吲哚-3-丁酸（IBA）的四分之一强度MS培养基能有效地诱导再生芽生根，在该培养基上可从再生芽中再生49根。将微繁殖的植物移植到含有土壤和椰子泥炭（1:1）混合物的花盆中，并在生长室中保持25±2°C、相对湿度80%、辐照度100µmol m−2 s−1的16小时光周期。八周后，植物被转移到田地里，在那里土壤与农家肥和沙子混合。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Herbs, Spices and Medicinal Plants Medicine-Complementary and Alternative Medicine

CiteScore

3.00

自引率

0.00%

发文量

期刊介绍： The Journal of Herbs, Spices & Medicinal Plants is an essential reference filled with recent research and other valuable information associated with herbs, spices, and medicinal plants. The Journal serves as a focus point through which investigators and others may publish material of importance to the production, marketing, and utilization of these plants and associated extracts. The journal covers the following topics: growth, development, horticulture, ecology, physiology, genetics, chemistry, and economics. Original articles, review articles, and book reviews provide information of interest to an international audience of researchers, teachers, technicians, and managers involved with production and/or marketing of herbs, spices, and medicinal plants. Managers of food companies, food processing facilities, medical research laboratories, government agencies, and others interested in new chemicals, food additives, international trade, patents, and other items can easily review new findings. The Journal of Herbs, Spices & Medicinal Plants is a forum in which recent research and other information associated with herbs, spices, and medicinal plants is shared. The Journal represents a centralized database accessible by investigators within the international community that work with or have an interest in herbs, spices, and medicinal plants.