A. Nowak, A. Klimowicz, Wiktoria Duchnik, Ł. Kucharski, Katarzyna Florkowska, Anna Muzykiewicz, D. Wira, Joanna Zielonkabrzezicka, Anita Siedłowska, Kinga Nadarzewska
{"title":"Application of green-extraction technique to evaluate of antioxidative capacity of wild population of fireweed (Epilobium angustifolium)","authors":"A. Nowak, A. Klimowicz, Wiktoria Duchnik, Ł. Kucharski, Katarzyna Florkowska, Anna Muzykiewicz, D. Wira, Joanna Zielonkabrzezicka, Anita Siedłowska, Kinga Nadarzewska","doi":"10.2478/hepo-2019-0022","DOIUrl":null,"url":null,"abstract":"Summary Introduction: Fireweed (Epilobium angustifolium (L.) Holub) is a common weed growing on meadows, roadside and agricultural wasteland, creating vast, rapidly spreading fields. Objective: The aim of the study was to evaluate the antioxidant activity of extracts from fresh fireweed, harvested at three ripening stages. Methods: Analysis of antioxidative activity was carried out by DPPH, ABTS and FRAP methods. Total polyphenol and total flavonoid content were also determined. Plant material was extracted using ultra-sound-assisted green extraction technique with methanol, ethanol and isopropanol at different concentrations and water. Results: The highest antioxidant activity evaluated by DPPH, ABTS and FRAP was found for the extracts prepared in 70% ethanol. The highest content of total polyphenols were observed in extracts in 70% ethanol, whereas the highest content of flavonoids extracts in undiluted methanol. Conclusion: Epilobium angustifolium harvested at fruit ripening stage seems to be a valuable source of antioxidants.","PeriodicalId":12990,"journal":{"name":"Herba Polonica","volume":"65 1","pages":"18 - 30"},"PeriodicalIF":0.0000,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"19","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Herba Polonica","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2478/hepo-2019-0022","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 19
Abstract
Summary Introduction: Fireweed (Epilobium angustifolium (L.) Holub) is a common weed growing on meadows, roadside and agricultural wasteland, creating vast, rapidly spreading fields. Objective: The aim of the study was to evaluate the antioxidant activity of extracts from fresh fireweed, harvested at three ripening stages. Methods: Analysis of antioxidative activity was carried out by DPPH, ABTS and FRAP methods. Total polyphenol and total flavonoid content were also determined. Plant material was extracted using ultra-sound-assisted green extraction technique with methanol, ethanol and isopropanol at different concentrations and water. Results: The highest antioxidant activity evaluated by DPPH, ABTS and FRAP was found for the extracts prepared in 70% ethanol. The highest content of total polyphenols were observed in extracts in 70% ethanol, whereas the highest content of flavonoids extracts in undiluted methanol. Conclusion: Epilobium angustifolium harvested at fruit ripening stage seems to be a valuable source of antioxidants.