Assessment of the Teratogenic Effect of Sulfadoxine-Pyrimethamine on the Chicken Embryo

IF 3.4 Q2 TOXICOLOGY

Journal of Toxicology Pub Date : 2022-03-15 DOI:10.1155/2022/2995492

Rachida Moussa Tari, Aboudoulatif Diallo, Emmanuelle Kouame, P. Assogba, Essotolom Badjabaissi, Lawson-evi Povi, B. Bakoma, Y. Potchoo, K. Tona

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引用次数: 0

Abstract

Background The sulfadoxine-pyrimethamine combination is a product used in the intermittent preventive treatment (IPT) of malaria in pregnant women in our country. To date, there is very little data on the teratogenic effect of this product. This study proposed to evaluate the teratogenic effect of sulfadoxine-pyrimethamine on chicken embryos. Methods The teratogenic effect of the product was evaluated on chicken embryos at a dose of 1.3 mg/g sulfadoxine and 0.06 mg/g pyrimethamine. The product was injected before the start of incubation and on days 12, 14, 16, and 18 of incubation. One batch received a double injection of the product on days 16 and 18 of incubation. The quality of the hatched chicks was evaluated by the Tona Score followed by the determination of hematological and biochemical parameters. Results From the aforementioned, it appears that the eggs treated with sulfadoxine-pyrimethamine significantly decreased the hatchability rate of the eggs. The chicks obtained were all of very good quality. Apart from a significant decrease in the weight of the chicks of the batch that received the injection twice and a significant increase in the weight of the yolk sac of the chicks of the batch that received the injection on day 16 compared to the control, no variation was obtained. A significant increase in the white blood cell count of the chicks compared to the control was reported in the chicks of the batch injected before incubation and on day 12, as well as a significant increase in the platelet count of the chicks of the batch injected twice. For biochemical parameters, no significant difference was reported in ALT and AST. Conclusion Sulfadoxine-pyrimethamine decreased egg hatch and caused an increase in embryo and chick mortality as well as a loss in relative chick weight and an increase in relative yolk sac weight. More in-depth studies would be needed on sulfadoxine-pyrimethamine teratogenicity and the benefit/risk ratio of this drug during pregnancy.

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磺胺嘧啶对鸡胚致畸作用的评价

背景磺胺多辛-乙胺嘧啶联合用药是我国用于孕妇疟疾间歇预防治疗的一种产品。迄今为止，关于该产品致畸作用的数据很少。本研究旨在评价磺胺多辛-乙胺嘧啶对鸡胚的致畸作用。方法以1.3 mg/g磺胺多辛和0.06 mg/g乙胺嘧啶为剂量，对鸡胚进行致畸作用评价。在孵育开始前和孵育的第12、14、16、18天注射本品。其中一批在孵育的第16天和第18天两次注射该产品。采用托纳评分法评价雏鸡的品质，并测定血液学和生化指标。结果综上所述，磺胺多辛-乙胺嘧啶处理显著降低了卵的孵化率。获得的雏鸡质量都很好。除了第16天注射两次的雏鸡体重显著下降和第16天注射的雏鸡卵黄囊重量显著增加外，其他均未见变化。据报道，孵育前和第12天注射的批次雏鸡的白细胞计数明显高于对照组，两次注射的批次雏鸡的血小板计数显著增加。在生化参数方面，ALT和AST无显著差异。结论磺胺多辛-乙胺嘧啶降低了鸡蛋的孵化率，增加了胚胎和鸡的死亡率，降低了鸡的相对体重，增加了卵黄囊的相对重量。需要对磺胺多辛-乙胺嘧啶致畸性和该药物在妊娠期间的获益/风险比进行更深入的研究。

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来源期刊

Journal of Toxicology TOXICOLOGY-

CiteScore

5.50

自引率

3.40%

发文量

审稿时长

10 weeks

期刊介绍： Journal of Toxicology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies in all areas of toxicological sciences. The journal will consider articles looking at the structure, function, and mechanism of agents that are toxic to humans and/or animals, as well as toxicological medicine, risk assessment, safety evaluation, and environmental health.