Bacterial Proliferation Reduces Sulphur Toxicity in Stabilization Ponds: Safer Water Resources by Photosynthesis

Abstract

Background: Studies suggest that sulfur may react with plants or monocellular organisms, such as fungi, to produce toxic agents. It has been theorized that sulfur enters cells and affects their respiration. This study reports on a phototroph development that leads to the diminution and/or production of sulfur and release of hydrogen sulfide from public ponds. Methods: This study was conducted in anoxic stabilization ponds at a dimension of 1×0.25×1 (m), with a hydraulic retention time of 6 days. The ponds’ water was tested during the dark and light cycles. The experiments were carried out by factorial design and measured three variables: volumetric organic loading, sulphate concentrations and temperature. Results: Cyanobacteria and Rhodobacter species were the most abundant phototroph cellular organisms. The mean value of total sulfur and un-ionized hydrogen sulfide concentration were 74 mg/L and 21 mg/L, respectively, at the volumetric organic loading of 100 g BOD5/m3 per day. The efficiencies of biochemical and chemical oxygen removal were 71.9% and 59.1%, respectively. The mean proliferation rate of photosynthetic sulfur bacteria was 3.4×106 cells/ml. Conclusion: photosynthetic sulfur bacteria significantly reduced the sulfur concentration generated in the ponds. The illumination of bright light enhanced the sulfur reduction by the photosynthetic sulfur bacteria in the wastewater of the anoxic stabilization ponds.