Mucoadhesive cellulose Nanocrystal-chitosan nanocomposite for the delivery of hydrophobic compounds

Q3 Materials Science

JCIS open Pub Date : 2023-10-01 DOI:10.1016/j.jciso.2023.100092

Dae Sung Kim , Fatemah Haji , Joseph Jun , Jiyoo Baek , Lian Han , Peter Wu , Brian Dixon , Kam C. Tam

{"title":"Mucoadhesive cellulose Nanocrystal-chitosan nanocomposite for the delivery of hydrophobic compounds","authors":"Dae Sung Kim , Fatemah Haji , Joseph Jun , Jiyoo Baek , Lian Han , Peter Wu , Brian Dixon , Kam C. Tam","doi":"10.1016/j.jciso.2023.100092","DOIUrl":null,"url":null,"abstract":"<div><p>Cellulose nanocrystals (CNC) have received much attention as a drug delivery vehicle, but their hydrophilic nature hinders hydrophobic drug loading. Ionotropic gelation using CNC and chitosan (CS) can enhance the loading/encapsulation capacity of hydrophobic compounds, improve colloidal stability, and strengthen mucoadhesion due to the cationic surface of CS. The colloidal behavior of CNC/CS nanocomposites loaded with emamectin benzoate (EMB) were elucidated by measuring the particle size, zeta potential, contact angle, and morphological structure using transmission electron microscopy. The mucoadhesive properties of the nanocomposites were evaluated by viscometric and titration method, followed by testing with zebrafish mucus. A facile and reproducible protocol to synthesize mucoadhesive CNC/CS nanocomposites that can encapsulate hydrophobic drugs is demonstrated. The optimal mass ratio for the synthesis was 1:10 (CS:CNC w/w), yielding the smallest average particle size (∼200 nm), highest zeta potentials (+40 mV), and highest drug encapsulation capacity (68.8 ± 8.7%). The steric stabilization effect of polyvinylpyrrolidone (PVP) and amphiphilic CNC stabilized the colloidal system. Importantly, the CS-coating technique enhanced the colloidal stability due to electrostatic intramolecular repulsion of the positive CS. CNC/CS nanocomposites exhibited enhanced mucoadhesive interaction with porcine mucin protein and live zebrafish mucus.</p></div>","PeriodicalId":73541,"journal":{"name":"JCIS open","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"JCIS open","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2666934X23000193","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Materials Science","Score":null,"Total":0}

引用次数: 0

Abstract

Cellulose nanocrystals (CNC) have received much attention as a drug delivery vehicle, but their hydrophilic nature hinders hydrophobic drug loading. Ionotropic gelation using CNC and chitosan (CS) can enhance the loading/encapsulation capacity of hydrophobic compounds, improve colloidal stability, and strengthen mucoadhesion due to the cationic surface of CS. The colloidal behavior of CNC/CS nanocomposites loaded with emamectin benzoate (EMB) were elucidated by measuring the particle size, zeta potential, contact angle, and morphological structure using transmission electron microscopy. The mucoadhesive properties of the nanocomposites were evaluated by viscometric and titration method, followed by testing with zebrafish mucus. A facile and reproducible protocol to synthesize mucoadhesive CNC/CS nanocomposites that can encapsulate hydrophobic drugs is demonstrated. The optimal mass ratio for the synthesis was 1:10 (CS:CNC w/w), yielding the smallest average particle size (∼200 nm), highest zeta potentials (+40 mV), and highest drug encapsulation capacity (68.8 ± 8.7%). The steric stabilization effect of polyvinylpyrrolidone (PVP) and amphiphilic CNC stabilized the colloidal system. Importantly, the CS-coating technique enhanced the colloidal stability due to electrostatic intramolecular repulsion of the positive CS. CNC/CS nanocomposites exhibited enhanced mucoadhesive interaction with porcine mucin protein and live zebrafish mucus.

Abstract Image

查看原文本刊更多论文

粘性纤维素-纳米晶体-壳聚糖纳米复合材料用于递送疏水性化合物

纤维素纳米晶体(CNC)作为一种药物递送载体受到了广泛的关注，但其亲水性阻碍了疏水性药物的装载。CNC和壳聚糖(CS)的亲电离性凝胶化可以增强疏水化合物的负载/封装能力，提高胶体稳定性，并且由于CS的阳离子表面而增强黏附。通过透射电镜对纳米复合材料粒径、zeta电位、接触角和形态结构的测定，研究了EMB的胶体行为。采用粘度法和滴定法评价了纳米复合材料的粘接性能，并用斑马鱼黏液进行了测试。展示了一种简单、可重复的合成可包封疏水药物的粘接CNC/CS纳米复合材料的方法。合成的最佳质量比为1:10 (CS:CNC w/w)，平均粒径最小(~ 200 nm)， zeta电位最高(+40 mV)，包封量最高(68.8±8.7%)。聚乙烯吡咯烷酮(PVP)和两亲性CNC的立体稳定作用稳定了胶体体系。重要的是，由于正CS的静电分子内排斥，CS涂层技术增强了胶体稳定性。CNC/CS纳米复合材料与猪黏蛋白和活斑马鱼黏液的黏附作用增强。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊