PATHOGENICITY OF ISOLATES OF ENTOMOPATHOGENIC FUNGI TO WORKERS OF Acromyrmex heyeri (Forel, 1899) (HYMENOPTERA: FORMICIDAE)

Q4 Environmental Science

Floresta Pub Date : 2023-01-18 DOI:10.5380/rf.v53i1.84838

M. A. Saldanha, E. C. Costa, M. Muniz, A. C. Quevedo, L. Pedron, J. S. Sarzi

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引用次数: 0

Abstract

Leaf-cutting ants stand out among the main pests of agricultural and forestry crops. Chemical control is the most used method to control these insect pests, requiring the development of techniques that are less harmful to the environment. The present study aimed at verifying the action of Beauveria bassiana (Bals.) Vuill, Cordyceps fumosorosea (Wize), and Metarhizium anisopliae (Metsch.) Sorokin isolates on the in vitro mortality of Acromyrmex heyeri (Forel). Seven isolates of entomopathogenic fungi were used: three isolates of Beauveria bassiana (IBCB 66, IBCB 170, and IBCB 632), one isolate of Cordyceps fumosorosea (IBCB 130), and three isolates of Metarhizium anisopliae (IBCB 348, IBCB 383, and IBCB 425), and five concentrations of spores (1.0×104, 1.0×105, 1.0×106, 1.0×107, and 1.0×108 spores mL-1) for each fungal isolate. The control treatment consisted of autoclaved distilled water and 0.01% Tween 40, without fungal pathogens. The experiments were carried out in a completely randomized design with five replications. Estimation of lethal concentrations of LC50 and LC90 was performed using Probit analysis. The isolates of B. bassiana, C. fumosorosea, and M. anisopliae were pathogenic to leaf-cutting ants with the five tested concentrations of spores. Higher mortality of the specimens was reached with increasing concentration. The entomopathogenic fungi evaluated in this study proved to be efficient agents for the biological control of Acromyrmex heyeri.

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昆虫病原真菌分离株对黑翅蠊（Forel，1899）工蜂的致病性（膜翅目：蚁科）

割叶蚁是农林作物的主要害虫之一。化学防治是控制这些害虫最常用的方法，需要开发对环境危害较小的技术。本研究旨在验证球孢白僵菌（Bals.）Vuill、烟曲霉（Wize）和绿僵菌（Metsch.）Sorokin分离株对Acromrmex heyeri（Forel）体外死亡率的影响。使用了7个昆虫病原真菌分离株：3个球孢白僵菌分离株（IBCB 66、IBCB 170和IBCB 632）、1个烟草虫草分离株（IBCB 130）和3个绿僵菌分离物（IBCB 348、IBCB 383和IBCB 425），每个真菌分离株的孢子浓度为5个（1.0×104、1.0×105、1.0×106、1.0×107和1.0×108孢子mL-1）。对照处理由高压灭菌蒸馏水和0.01%吐温40组成，不含真菌病原体。实验是在一个完全随机的设计中进行的，有五个重复。使用Probit分析对LC50和LC90的致死浓度进行估计。在五种测试浓度的孢子中，球孢B.bassiana、烟曲霉C.fumosorosea和M.anisopliae的分离株对切叶蚁具有致病性。随着浓度的增加，标本的死亡率越来越高。本研究评价的昆虫病原真菌被证明是有效的生物防治剂。

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来源期刊

Floresta Environmental Science-Nature and Landscape Conservation

CiteScore

0.80

自引率

0.00%

发文量

期刊介绍： Revista Floresta admits articles of scientific contribution in Forest Science, in the areas of Nature Conservation, Economy and Forest Policy, Forest Management, Silviculture and Wood Technology. The main task is to promote and divulgate the development of Forest Science in Brazil and in the world. Articles published in Revista Floresta are under total responsibility of the authors, who are aware that they are original studies. It remains implicit that articles were not submitted for publication to any other divulgation media. It remains explicit the agreement of authors to norms of the journal and, along the development of their work, the observance of ethic aspects and respect of the current copyright legislation.