Faecal microbiota transplantation is better than probiotics for tissue regeneration of type 2 diabetes mellitus injuries in mice.

IF 2.5 4区医学 Q3 ENDOCRINOLOGY & METABOLISM

Archives of Physiology and Biochemistry Pub Date : 2024-06-01 Epub Date: 2022-06-08 DOI:10.1080/13813455.2022.2080229

Yuying Wang, Zhenpeng Yang, Huazhen Tang, Xibo Sun, Jinxiu Qu, Shuai Lu, Benqiang Rao

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引用次数: 0

Abstract

Context: Western diet and unhealthy lifestyle have contributed to the continued growth of type 2 diabetes mellitus (T2DM). T2DM is associated with dysbacteriosis, and studies have found that altering the gut microbiota has a positive effect on treatment.

Objective: In addition to hyperglycaemia, T2DM often causes damage to multiple organs. However, there are few studies on organ damage from faecal microbiota transplantation (FMT).

Materials and methods: T2DM mice were divided into four groups and were given phosphate buffered saline (PBS) (T2DM group), FMT (FMT group), Lactobacillus (LAB group), and Bifidobacterium (BIO group) by gavage for six weeks, respectively. Mice on a normal diet (control group) were gavaged with PBS for six weeks.

Results: After gavage treatment, FMT, LAB, and BIO groups were similar in lowering glucose, endotoxemia was slightly reduced, and the colonic mucus layer and liver lobules developed towards normal tissue. Surprisingly, we found that the FMT group had unique effects on islet cell regeneration, increased functional β cells, and insulin sensitivity.

Discussion and conclusion: Lactobacillus has the best glucose-lowering effect, but FMT has obvious advantages in β-cell regeneration, which provides new treatment ideas for tissue damage caused by T2DM.

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粪便微生物群移植对2型糖尿病小鼠损伤的组织再生优于益生菌。

西方饮食和不健康的生活方式导致了2型糖尿病（T2DM）的持续增长。T2DM与菌群失调有关，研究发现，改变肠道微生物群对治疗有积极影响。目的除了高血糖外，2型糖尿病还经常对多个器官造成损害。然而，很少有关于粪便微生物群移植（FMT）对器官损伤的研究。材料和方法2DM小鼠被分为四组，分别灌胃给予磷酸盐缓冲盐水（PBS）（T2DM组）、FMT（FMT组）、乳酸杆菌（LAB组）和双歧杆菌（BIO组）6个月周。正常饮食的小鼠（对照组）用PBS灌胃6 周。结果经灌胃治疗后，FMT、LAB和BIO组的降血糖效果相似，内毒素血症略有减轻，结肠粘液层和肝小叶向正常组织发展。令人惊讶的是，我们发现FMT组对胰岛细胞再生、功能性β细胞增加和胰岛素敏感性具有独特的影响。讨论与结论乳酸杆菌降血糖效果最好，但FMT在β细胞再生方面具有明显优势，为T2DM引起的组织损伤提供了新的治疗思路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Archives of Physiology and Biochemistry ENDOCRINOLOGY & METABOLISM-PHYSIOLOGY

CiteScore

6.90

自引率

3.30%

发文量

期刊介绍： Archives of Physiology and Biochemistry: The Journal of Metabolic Diseases is an international peer-reviewed journal which has been relaunched to meet the increasing demand for integrated publication on molecular, biochemical and cellular aspects of metabolic diseases, as well as clinical and therapeutic strategies for their treatment. It publishes full-length original articles, rapid papers, reviews and mini-reviews on selected topics. It is the overall goal of the journal to disseminate novel approaches to an improved understanding of major metabolic disorders. The scope encompasses all topics related to the molecular and cellular pathophysiology of metabolic diseases like obesity, type 2 diabetes and the metabolic syndrome, and their associated complications. Clinical studies are considered as an integral part of the Journal and should be related to one of the following topics: -Dysregulation of hormone receptors and signal transduction -Contribution of gene variants and gene regulatory processes -Impairment of intermediary metabolism at the cellular level -Secretion and metabolism of peptides and other factors that mediate cellular crosstalk -Therapeutic strategies for managing metabolic diseases Special issues dedicated to topics in the field will be published regularly.