Distribution of Salmonella virulence factors originated from sheep and cattle in Algerian slaughterhouses

IF 0.4 4区农林科学 Q4 VETERINARY SCIENCES

Journal of the Hellenic Veterinary Medical Society Pub Date : 2023-01-25 DOI:10.12681/jhvms.29148

S. Nouichi, L. Mezali, TM Hamdi

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引用次数: 0

Abstract

The present study was carried out to determine the virulence gene profiles associated with Salmonella subsp. enterica strains recovered from cattle and sheep samples by polymerase chain reaction (PCR). A total of 84 Salmonella isolates belonging to 10 serovars were screened for presence of 8 different virulence genes (invA, pefA, sefA, pipB, sseC, ssaP, spvC and iroB) using their specific primers. Different distribution patterns of these genes were noticed amongst the isolates. While the iroB gene was the most identified (n= 65, 77.4%), the sefA gene was not detected in all tested strains. pefA and spvC genes were detected in S. Typhimurium only with a rate of 3.6%. The four other genes were found to be present in 61.9% each. Occurrence of virulence genes did not vary significantly (P> 0.05) by serovar or animal species, neither by type of sample. The presence of all screened genes was significantly associated with resistance to at least one antibiotic from different classes except quinolones. These results show that transmissible pathogenic Salmonella strains are circulating in slaughterhouses in Algeria.

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产自阿尔及利亚屠宰场羊和牛的沙门氏菌毒力因子分布

本研究旨在确定与沙门氏菌亚种相关的毒力基因谱。采用聚合酶链式反应(PCR)从牛和羊样品中检出肠炎菌株。利用特异性引物对10个血清型84株沙门氏菌进行了8种不同毒力基因(invA、pefA、sefA、pipB、sseC、ssaP、spvC和iroB)的筛选。这些基因在不同的分离株中有不同的分布模式。iroB基因检出最多(n= 65, 77.4%)， sefA基因未检出。鼠伤寒沙门氏菌中pefA和spvC基因检出率仅为3.6%。其他四种基因各占61.9%。毒力基因的发生在不同的血清型和动物种类之间无显著差异(P> 0.05)，在不同的样品类型之间无显著差异。除喹诺酮类药物外，所有筛选基因的存在与对至少一种不同类别抗生素的耐药性显著相关。这些结果表明，传染性致病性沙门氏菌菌株在阿尔及利亚的屠宰场中流行。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of the Hellenic Veterinary Medical Society VETERINARY SCIENCES-

CiteScore

0.60

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The Journal of the Hellenic Veterinary Medical Society (J Hellenic Vet Med Soc) is a quarterly peer-reviewed journal that publishes articles in all aspects of veterinary science and related disciplines. It is published by the Hellenic Veterinary Medical Society and is indexed in the Web of Science and in Scopus. There are no publication fees in the journal. Authors considering submitting manuscripts for evaluation and publication are requested to read carefully the instructions for authors and fully comply with them. Non-complying manuscripts may be returned to the corresponding author for formatting.