Novel NF-Y genes expressed during somatic embryogenesis in Medicago truncatula

IF 2.2 Q3 GENETICS & HEREDITY

Plant Gene Pub Date : 2022-09-01 DOI:10.1016/j.plgene.2022.100364

Elina Potsenkovskaia , Varvara Tvorogova , Daria Yakovleva , Natalia Zlydneva , Ludmila Lutova

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引用次数: 1

Abstract

Somatic embryogenesis is widely used in biotechnology as a regeneration process conditioning many protocols for plant transformation and in vitro propagation. The main objective of this study was to search for new potential somatic embryogenesis regulators among the genes belonging to the NF-Y family and to analyse the mechanism of their action in Medicago truncatula. We found that two LEC1-type NF-YB genes, MtNF-YB3 and 10, are highly expressed in the calli of the embryogenic 2HA line during somatic embryogenesis in M. truncatula. Overexpression of these genes did not have a significant effect on the somatic embryogenesis capacity. Several genes from NF-YA and C groups with high expression levels during somatic embryogenesis were found. We demonstrated that transcription factors, encoded by some of them, were able to interact with MtNF-YB3 and 10, and overexpression of one of these genes, MtNF-YA3, increased callus weight. The results obtained provide new information about NF-Y genes functioning during plant regeneration and can be used for the search of new somatic embryogenesis stimulants.

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短叶苜蓿体细胞胚胎发生过程中NF-Y新基因的表达

体细胞胚胎发生作为一种再生过程被广泛应用于生物技术中，它决定了许多植物转化和离体繁殖的方案。本研究的主要目的是在NF-Y家族基因中寻找新的潜在的体细胞胚胎发生调控因子，并分析其在凤头苜蓿中的作用机制。我们发现两个lec1型NF-YB基因，MtNF-YB3和10，在胚性2HA细胞系的愈伤组织中高表达。这些基因的过表达对体细胞胚发生能力无显著影响。在体细胞胚发生过程中发现了NF-YA和C组中几个高表达的基因。我们证明了由其中一些基因编码的转录因子能够与MtNF-YB3和10相互作用，并且其中一个基因MtNF-YA3的过表达会增加愈伤组织的重量。这些结果为NF-Y基因在植物再生过程中的功能提供了新的信息，并可用于寻找新的体细胞胚胎发生刺激剂。

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来源期刊

Plant Gene Agricultural and Biological Sciences-Plant Science

CiteScore

4.50

自引率

0.00%

发文量

审稿时长

51 days

期刊介绍： Plant Gene publishes papers that focus on the regulation, expression, function and evolution of genes in plants, algae and other photosynthesizing organisms (e.g., cyanobacteria), and plant-associated microorganisms. Plant Gene strives to be a diverse plant journal and topics in multiple fields will be considered for publication. Although not limited to the following, some general topics include: Gene discovery and characterization, Gene regulation in response to environmental stress (e.g., salinity, drought, etc.), Genetic effects of transposable elements, Genetic control of secondary metabolic pathways and metabolic enzymes. Herbal Medicine - regulation and medicinal properties of plant products, Plant hormonal signaling, Plant evolutionary genetics, molecular evolution, population genetics, and phylogenetics, Profiling of plant gene expression and genetic variation, Plant-microbe interactions (e.g., influence of endophytes on gene expression; horizontal gene transfer studies; etc.), Agricultural genetics - biotechnology and crop improvement.