Occurrence of cherry viruses in South Tyrol (Italy) by comparing growth periods in two consecutive years

IF 1.9 3区农林科学 Q2 AGRONOMY

Phytopathologia Mediterranea Pub Date : 2022-09-15 DOI:10.36253/phyto-12807

Evi Deltedesco, Magdalena Niedrist, S. Oettl

引用次数: 0

Abstract

Sweet cherries (Prunus avium L.) are important as fruit crops, and can be affected by numerous viruses. An investigation on the occurrence of the three most common viruses of sweet cherry was carried out in commercially managed orchards in South Tyrol (Italy). The incidence of apple chlorotic leaf spot virus (ACLSV), Prunus necrotic ringspot virus (PNRSV) and prune dwarf virus (PDV) was investigated using enzyme-linked immunosorbent assays (ELISA) and the reverse transcriptase-polymerase chain reaction technique (RT-PCR) in spring 2018 and 2020, and during the summer and autumn of 2020. All three viruses were detected in the surveyed orchards. Comparative analyses showed that detection was more effective with RT-PCR than with ELISA, especially for detecting PNRSV and PDV. Mixed infections were detected in all the surveyed orchards. The results also showed clear differences between and during host growth periods, likely due to a variable virus concentration in the host trees.

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意大利南蒂罗尔州樱桃病毒连续两年生长期的比较

甜樱桃(Prunus avium L.)是重要的水果作物，可以受到许多病毒的影响。在南蒂罗尔(意大利)的商业管理果园中对甜樱桃最常见的三种病毒的发生情况进行了调查。采用酶联免疫吸附试验(ELISA)和逆转录聚合酶链反应技术(RT-PCR)研究了2018年春季和2020年夏季和秋季苹果绿斑病毒(ACLSV)、李子坏死环斑病毒(PNRSV)和李子矮秆病毒(PDV)的发病情况。在调查的果园中均检测到这三种病毒。对比分析表明，RT-PCR检测PNRSV和PDV的效果明显优于ELISA。所有果园均存在混合侵染。结果还显示了寄主生长期间和寄主生长期间的明显差异，可能是由于寄主树中不同的病毒浓度所致。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Phytopathologia Mediterranea 生物-植物科学

CiteScore

4.40

自引率

8.30%

发文量

审稿时长

6-12 weeks

期刊介绍： Phytopathologia Mediterranea is an international journal edited by the Mediterranean Phytopathological Union. The journal’s mission is the promotion of plant health for Mediterranean crops, climate and regions, safe food production, and the transfer of new knowledge on plant diseases and their sustainable management. The journal deals with all areas of plant pathology, including etiology, epidemiology, disease control, biochemical and physiological aspects, and utilization of molecular technologies. All types of plant pathogens are covered, including fungi, oomycetes, nematodes, protozoa, bacteria, phytoplasmas, viruses, and viroids. The journal also gives a special attention to research on mycotoxins, biological and integrated management of plant diseases, and the use of natural substances in disease and weed control. The journal focuses on pathology of Mediterranean crops grown throughout the world. The Editorial Board of Phytopathologia Mediterranea has recently been reorganised, under two Editors-in-Chief and with an increased number of editors.